This research is aimed at isolating, characterising and selecting cellulolytic bacteria from raw composting materials consisting of wastes from food industry of vegetable. A total of 180 isolates were screened for their cellulosolytic activity and characterized by phenotypic and molecular methods. Moreover, intraspecific DNA polymorphism was evaluated. Time-courses of cellulase production were also analysed by CMC and filter paper activity assays. Only microbial strains exhibiting the highest cellulolytic activity in liquid medium were, then, evaluated for their hydrolytic activity on different soluble and insoluble substrates and cellulase production regulation.
Selection of cellulolytic bacteria for processing of cellulosic biomass / Ventorino, Valeria; Amore, A.; Faraco, Vincenza; Blaiotta, Giuseppe; Pepe, Olimpia. - In: JOURNAL OF BIOTECHNOLOGY. - ISSN 0168-1656. - 150:(2010), pp. 181-181. [10.1016/j.jbiotec.2010.08.471]
Selection of cellulolytic bacteria for processing of cellulosic biomass.
VENTORINO, VALERIA;FARACO, VINCENZA;BLAIOTTA, GIUSEPPE;PEPE, OLIMPIA
2010
Abstract
This research is aimed at isolating, characterising and selecting cellulolytic bacteria from raw composting materials consisting of wastes from food industry of vegetable. A total of 180 isolates were screened for their cellulosolytic activity and characterized by phenotypic and molecular methods. Moreover, intraspecific DNA polymorphism was evaluated. Time-courses of cellulase production were also analysed by CMC and filter paper activity assays. Only microbial strains exhibiting the highest cellulolytic activity in liquid medium were, then, evaluated for their hydrolytic activity on different soluble and insoluble substrates and cellulase production regulation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
