Since the possibility to biotechnologically produce melanin by Streptomycetes using plant biomass has been so far poorly investigated, Posidonia oceanica egagropili, a marine waste accumulating along the Mediterranean Sea coasts, was explored as a renewable source to enhance extracellular melanin production by Streptomyces roseochromogenes ATCC 13400. Therefore, different amounts of egagropili powder were added to a culture medium containing glucose, malt extract, and yeast extract, and their effect on the melanin biosynthesis was evaluated. A 2.5 g·L-1 supplementation in 120-h shake flask growths at 26 °C, at pH 6.0 and 250 rpm, was found to enhance the melanin production up to 3.94 ± 0.12 g·L-1, a value 7.4-fold higher than the control. Moreover, 2-L batches allowed to reach a concentration of 9.20 ± 0.12 g·L-1 in 96 h with a productivity of 0.098 g·L-1·h-1. Further studies also demonstrated that the melanin production enhancement was due to the synergistic effect of both the lignin carbohydrate complex and the holocellulose components of the egagropili. Finally, the pigment was purified from the broth supernatant by acidic precipitation and reversed-phase chromatography, characterized by UV absorbance and one- and two-dimensional NMR, and also tested for its chemical, antioxidant, and photo-protective properties. KEY POINTS: • S. roseochromogenes ATCC 13400 produces extracellular soluble melanin. • Egagropili added to the growth medium enhances melanin production and productivity. • Both the lignin carbohydrate complex and the holocellulose egagropili components influence the melanin biosynthesis.

Enhanced Streptomyces roseochromogenes melanin production by using the marine renewable source Posidonia oceanica egagropili / Restaino, Odile Francesca; Scognamiglio, Monica; Mirpoor, Seyedeh Fatemeh; Cammarota, Marcella; Ventriglia, Riccardo; Giosafatto, CONCETTA VALERIA LUCIA; Fiorentino, Antonio; Porta, Raffaele; Schiraldi, Chiara. - In: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. - ISSN 0175-7598. - 106:21(2022), pp. 7265-7283. [10.1007/s00253-022-12191-8]

Enhanced Streptomyces roseochromogenes melanin production by using the marine renewable source Posidonia oceanica egagropili

Restaino, Odile Francesca;Mirpoor, Seyedeh Fatemeh;Giosafatto Concetta Valeria Lucia;Fiorentino, Antonio;Porta, Raffaele;Schiraldi, Chiara
2022

Abstract

Since the possibility to biotechnologically produce melanin by Streptomycetes using plant biomass has been so far poorly investigated, Posidonia oceanica egagropili, a marine waste accumulating along the Mediterranean Sea coasts, was explored as a renewable source to enhance extracellular melanin production by Streptomyces roseochromogenes ATCC 13400. Therefore, different amounts of egagropili powder were added to a culture medium containing glucose, malt extract, and yeast extract, and their effect on the melanin biosynthesis was evaluated. A 2.5 g·L-1 supplementation in 120-h shake flask growths at 26 °C, at pH 6.0 and 250 rpm, was found to enhance the melanin production up to 3.94 ± 0.12 g·L-1, a value 7.4-fold higher than the control. Moreover, 2-L batches allowed to reach a concentration of 9.20 ± 0.12 g·L-1 in 96 h with a productivity of 0.098 g·L-1·h-1. Further studies also demonstrated that the melanin production enhancement was due to the synergistic effect of both the lignin carbohydrate complex and the holocellulose components of the egagropili. Finally, the pigment was purified from the broth supernatant by acidic precipitation and reversed-phase chromatography, characterized by UV absorbance and one- and two-dimensional NMR, and also tested for its chemical, antioxidant, and photo-protective properties. KEY POINTS: • S. roseochromogenes ATCC 13400 produces extracellular soluble melanin. • Egagropili added to the growth medium enhances melanin production and productivity. • Both the lignin carbohydrate complex and the holocellulose egagropili components influence the melanin biosynthesis.
2022
Enhanced Streptomyces roseochromogenes melanin production by using the marine renewable source Posidonia oceanica egagropili / Restaino, Odile Francesca; Scognamiglio, Monica; Mirpoor, Seyedeh Fatemeh; Cammarota, Marcella; Ventriglia, Riccardo; Giosafatto, CONCETTA VALERIA LUCIA; Fiorentino, Antonio; Porta, Raffaele; Schiraldi, Chiara. - In: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. - ISSN 0175-7598. - 106:21(2022), pp. 7265-7283. [10.1007/s00253-022-12191-8]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/902249
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