The metabolic profile of T-2 toxin (T-2) and its modified form T-2-3-glucoside (T-2-3-Glc) remain unexplored in human samples. Therefore, the present study aimed to investigate the presence of T-2, T-2-3-Glc and their respective major metabolites in human urine samples (n = 300) collected in South Italy through an ultra-high performance liquid chromatography (UHPLC) coupled to Q-Orbitrap-HRMS methodology. T-2 was quantified in 21% of samples at a mean concentration of 1.34 ng/mg Crea (range: 0.22–6.54 ng/mg Crea). Almost all the major T-2 metabolites previously characterized in vitro were tentatively found, remarking the occurrence of 3′-OH-T-2 (99.7%), T-2 triol (56%) and HT-2 (30%). Regarding T-2-3-Glc, a low prevalence of the parent mycotoxin (1%) and its metabolites were observed, with HT-2-3-Glc (17%) being the most prevalent compound, although hydroxylated products were also detected. Attending to the large number of testing positive for T-2 or its metabolites, this study found a frequent exposure in Italian population.

Human biomonitoring of t-2 toxin, t-2 toxin-3-glucoside and their metabolites in urine through high-resolution mass spectrometry / Narvaez, A.; Izzo, L.; Pallares, N.; Castaldo, L.; Rodriguez-Carrasco, Y.; Ritieni, A.. - In: TOXINS. - ISSN 2072-6651. - 13:12(2021), p. 869. [10.3390/toxins13120869]

Human biomonitoring of t-2 toxin, t-2 toxin-3-glucoside and their metabolites in urine through high-resolution mass spectrometry

Izzo L.
Primo
;
Ritieni A.
Ultimo
2021

Abstract

The metabolic profile of T-2 toxin (T-2) and its modified form T-2-3-glucoside (T-2-3-Glc) remain unexplored in human samples. Therefore, the present study aimed to investigate the presence of T-2, T-2-3-Glc and their respective major metabolites in human urine samples (n = 300) collected in South Italy through an ultra-high performance liquid chromatography (UHPLC) coupled to Q-Orbitrap-HRMS methodology. T-2 was quantified in 21% of samples at a mean concentration of 1.34 ng/mg Crea (range: 0.22–6.54 ng/mg Crea). Almost all the major T-2 metabolites previously characterized in vitro were tentatively found, remarking the occurrence of 3′-OH-T-2 (99.7%), T-2 triol (56%) and HT-2 (30%). Regarding T-2-3-Glc, a low prevalence of the parent mycotoxin (1%) and its metabolites were observed, with HT-2-3-Glc (17%) being the most prevalent compound, although hydroxylated products were also detected. Attending to the large number of testing positive for T-2 or its metabolites, this study found a frequent exposure in Italian population.
2021
Human biomonitoring of t-2 toxin, t-2 toxin-3-glucoside and their metabolites in urine through high-resolution mass spectrometry / Narvaez, A.; Izzo, L.; Pallares, N.; Castaldo, L.; Rodriguez-Carrasco, Y.; Ritieni, A.. - In: TOXINS. - ISSN 2072-6651. - 13:12(2021), p. 869. [10.3390/toxins13120869]
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Descrizione: Human Biomonitoring of T-2 Toxin, T-2 Toxin-3-Glucoside and Their Metabolites in Urine through High-Resolution Mass Spectrometry
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/879083
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