Enniatins (Enns) are mycotoxins produced by Fusarium spp. and are widely distributed contaminants of cereals and derivate products. Among the different identified enniatins, Enn B is the most relevant analogue in cereals in Europe. Therefore, the aim of this study was to investigate for the first time the occurrence of Enn B and Enn B phase I metabolites in 300 human urine samples throughout an ultrahigh-performance liquid chromatography high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) methodology. Three different sample preparation procedures were evaluated and salting-out liquid-liquid extraction showed satisfactory validation results. Enn B was quantified in 83.7% of samples ranging from 0.006 to 0.391 ng/mL (average content: 0.016 ng/mL). In line with the in vitro observations with human liver microsomes, in the here analyzed samples the Enn B monooxygenated, N-demethylated and dioxygenated metabolites were tentatively found in 87.7%, 96.3% and 6.7% of samples. The data of this pilot biomonitoring survey indicate a frequent intake of enniatins in Italy, supporting further toxicological studies to provide better basis for understanding their potential effects in humans.

Urinary levels of enniatin B and its phase I metabolites: First human pilot biomonitoring study / Rodríguez-Carrascoa, Yelko; Izzo, Luana; Gaspari, Anna; Graziani, Giulia; Mañes, Jordi; Ritieni, Alberto. - In: FOOD AND CHEMICAL TOXICOLOGY. - ISSN 0278-6915. - 118:(2018), pp. 454-459. [10.1016/j.fct.2018.05.052]

Urinary levels of enniatin B and its phase I metabolites: First human pilot biomonitoring study

Luana Izzo
Secondo
;
Alberto Ritieni
Ultimo
2018

Abstract

Enniatins (Enns) are mycotoxins produced by Fusarium spp. and are widely distributed contaminants of cereals and derivate products. Among the different identified enniatins, Enn B is the most relevant analogue in cereals in Europe. Therefore, the aim of this study was to investigate for the first time the occurrence of Enn B and Enn B phase I metabolites in 300 human urine samples throughout an ultrahigh-performance liquid chromatography high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) methodology. Three different sample preparation procedures were evaluated and salting-out liquid-liquid extraction showed satisfactory validation results. Enn B was quantified in 83.7% of samples ranging from 0.006 to 0.391 ng/mL (average content: 0.016 ng/mL). In line with the in vitro observations with human liver microsomes, in the here analyzed samples the Enn B monooxygenated, N-demethylated and dioxygenated metabolites were tentatively found in 87.7%, 96.3% and 6.7% of samples. The data of this pilot biomonitoring survey indicate a frequent intake of enniatins in Italy, supporting further toxicological studies to provide better basis for understanding their potential effects in humans.
2018
Urinary levels of enniatin B and its phase I metabolites: First human pilot biomonitoring study / Rodríguez-Carrascoa, Yelko; Izzo, Luana; Gaspari, Anna; Graziani, Giulia; Mañes, Jordi; Ritieni, Alberto. - In: FOOD AND CHEMICAL TOXICOLOGY. - ISSN 0278-6915. - 118:(2018), pp. 454-459. [10.1016/j.fct.2018.05.052]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/878657
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