The quantification of protein bound Maillard reaction products (MRPs) is still a challenge in food chemistry. Protein hydrolysis is the bottleneck step: it is time consuming and the protein degradation is not always complete. In this study, the quantitation of free amino acids and Amadori products (APs) was compared to the percentage of blocked lysine by using chemometric tools. Eighty thermally treated soybean samples were analyzed by mass spectrometry to measure the concentration of free amino acids, free APs and the protein-bound markers of the Maillard reaction (furosine, Ne-(carboxymethyl)-L-lysine, Ne-(carboxyethyl)-L-lysine, total lysine). Results demonstrated that Discriminant Analysis (DA) and Correlated Component Regression (CCR) correctly estimated the percent of blocked lysine in a validation and prediction set. These findings indicate that the measure of free markers reflects the extent of protein damage in soybean samples and it suggests the possibility to obtain rapid information on the quality of the industrial processes.

The quantification of free Amadori compounds and amino acids allows to model the bound Maillard reaction products formation in soybean products / Troise, Antonio Dario; Wiltafsky, Markus; Fogliano, Vincenzo; Vitaglione, Paola. - In: FOOD CHEMISTRY. - ISSN 0308-8146. - 247:(2018), pp. 29-38. [10.1016/j.foodchem.2017.12.019]

The quantification of free Amadori compounds and amino acids allows to model the bound Maillard reaction products formation in soybean products

Troise, Antonio Dario
Conceptualization
;
Vitaglione, Paola
Writing – Review & Editing
2018

Abstract

The quantification of protein bound Maillard reaction products (MRPs) is still a challenge in food chemistry. Protein hydrolysis is the bottleneck step: it is time consuming and the protein degradation is not always complete. In this study, the quantitation of free amino acids and Amadori products (APs) was compared to the percentage of blocked lysine by using chemometric tools. Eighty thermally treated soybean samples were analyzed by mass spectrometry to measure the concentration of free amino acids, free APs and the protein-bound markers of the Maillard reaction (furosine, Ne-(carboxymethyl)-L-lysine, Ne-(carboxyethyl)-L-lysine, total lysine). Results demonstrated that Discriminant Analysis (DA) and Correlated Component Regression (CCR) correctly estimated the percent of blocked lysine in a validation and prediction set. These findings indicate that the measure of free markers reflects the extent of protein damage in soybean samples and it suggests the possibility to obtain rapid information on the quality of the industrial processes.
2018
The quantification of free Amadori compounds and amino acids allows to model the bound Maillard reaction products formation in soybean products / Troise, Antonio Dario; Wiltafsky, Markus; Fogliano, Vincenzo; Vitaglione, Paola. - In: FOOD CHEMISTRY. - ISSN 0308-8146. - 247:(2018), pp. 29-38. [10.1016/j.foodchem.2017.12.019]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/701176
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