In the last decade, the benthic dinoflagellate Ostreopsis ovata has caused severe sanitary emergencies along the Mediterranean coasts of Europe. Liquid chromatography-mass spectrometry (LC-MS) played a key role in the identification of palytoxin as the parent toxin produced by O. ovata (Ciminiello et al., 2006). Palytoxin is one of the most potent non-protein marine toxins so far known (Deeds and Schwartz, 2010) as well as one of the most complex non-polymeric water-soluble molecules ever described (Ciminiello et al., 2011). Following this finding, a number of palytoxin analogues, that we named ovatoxins (a-f), have been found and identified basing on high resolution (HR) LC-MS evidence (Ciminiello et al., 2006, 2010). Among them, ovatoxin-a is the major component of O. ovata toxin profile, representing up to 55% of the total toxin content, with the remaining 46% being constituted by the whole of the other ovatoxins, and palytoxin being present in very minute amounts . Blooms of Ostreopsis spp. may result in ovatoxin contamination of seafood and, in order to prevent sanitary risks, the need exists to develop efficient extraction procedures to be coupled to rapid and sensitive monitoring methods of palytoxin-like compounds in seafood. In the present study, the best conditions for extraction of palytoxin-like compounds from seafood and their quantification by using liquid HR LC-MS will be presented.

High Resolution LC-MS of ovatoxins: a strategy to face an emerging threat to seafood consumers in the Mediterranean area / Dell'Aversano, Carmela; Ciminiello, Patrizia; DELLO IACOVO, Emma; Fattorusso, Ernesto; Forino, Martino; Tartaglione, Luciana. - (2012). (Intervento presentato al convegno PittCon Conference & Expo tenutosi a Orlando, Fl, USA nel 11-15 March 2012).

High Resolution LC-MS of ovatoxins: a strategy to face an emerging threat to seafood consumers in the Mediterranean area

DELL'AVERSANO, CARMELA;CIMINIELLO, PATRIZIA;DELLO IACOVO, EMMA;FATTORUSSO, ERNESTO;FORINO, MARTINO;TARTAGLIONE, LUCIANA
2012

Abstract

In the last decade, the benthic dinoflagellate Ostreopsis ovata has caused severe sanitary emergencies along the Mediterranean coasts of Europe. Liquid chromatography-mass spectrometry (LC-MS) played a key role in the identification of palytoxin as the parent toxin produced by O. ovata (Ciminiello et al., 2006). Palytoxin is one of the most potent non-protein marine toxins so far known (Deeds and Schwartz, 2010) as well as one of the most complex non-polymeric water-soluble molecules ever described (Ciminiello et al., 2011). Following this finding, a number of palytoxin analogues, that we named ovatoxins (a-f), have been found and identified basing on high resolution (HR) LC-MS evidence (Ciminiello et al., 2006, 2010). Among them, ovatoxin-a is the major component of O. ovata toxin profile, representing up to 55% of the total toxin content, with the remaining 46% being constituted by the whole of the other ovatoxins, and palytoxin being present in very minute amounts . Blooms of Ostreopsis spp. may result in ovatoxin contamination of seafood and, in order to prevent sanitary risks, the need exists to develop efficient extraction procedures to be coupled to rapid and sensitive monitoring methods of palytoxin-like compounds in seafood. In the present study, the best conditions for extraction of palytoxin-like compounds from seafood and their quantification by using liquid HR LC-MS will be presented.
2012
High Resolution LC-MS of ovatoxins: a strategy to face an emerging threat to seafood consumers in the Mediterranean area / Dell'Aversano, Carmela; Ciminiello, Patrizia; DELLO IACOVO, Emma; Fattorusso, Ernesto; Forino, Martino; Tartaglione, Luciana. - (2012). (Intervento presentato al convegno PittCon Conference & Expo tenutosi a Orlando, Fl, USA nel 11-15 March 2012).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/433128
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