In this work, we present an advanced fluorescence assay for the detection of traces of ocratoxin A and neomycin in food. The described assay is based on measurement of the fluctuations of the fluorescein-labeled analytes by a focused laser beam in the absence and in the presence of the specific antibodies anti-analytes. A competitive assay based on the utilization of unlabeled analytes was developed. The obtained results indicated that the combination of high-avidity IgG antibodies together with an innovative fluorescence immunoassay strategy resulted in the detection limit of 0.0078 ng and 0.0156 ng for ochratoxin A and neomycin, respectively.
FCS-besed sensing for the detection of ochratoxin and neomycin in food / Varriale, A.; Staiano, M.; Iozzino, L.; Severino, Lorella; Anastasio, Aniello; Cortesi, MARIA LUISA; D'Auria, S.. - In: PROTEIN AND PEPTIDE LETTERS. - ISSN 0929-8665. - STAMPA. - 16:12(2009), pp. 1425-1428.
FCS-besed sensing for the detection of ochratoxin and neomycin in food
SEVERINO, LORELLA;ANASTASIO, ANIELLO;CORTESI, MARIA LUISA;
2009
Abstract
In this work, we present an advanced fluorescence assay for the detection of traces of ocratoxin A and neomycin in food. The described assay is based on measurement of the fluctuations of the fluorescein-labeled analytes by a focused laser beam in the absence and in the presence of the specific antibodies anti-analytes. A competitive assay based on the utilization of unlabeled analytes was developed. The obtained results indicated that the combination of high-avidity IgG antibodies together with an innovative fluorescence immunoassay strategy resulted in the detection limit of 0.0078 ng and 0.0156 ng for ochratoxin A and neomycin, respectively.| File | Dimensione | Formato | |
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