The unnatural amino acid lysinoalanine (LAL) has been identified in milk and cheese products by liquid chromatography mass spectrometry (LC/ESI/MS) with selective ion monitoring (SIM) of the 9-fluorenyl-methylchloro-formate (FMOC) derivative. LAL is not present in raw milk or derived from Mozzarella cheese; however, high amounts of LAL are found in calcium caseinate and milk powder. As expected, milk fortified with caseinate or whey protein powder produces cheese with higher LAL content. Our analytical procedure is based on the simultaneous detection of specific ion masses of the FMOC–LAL derivative and the N-ε-methyl-lysine internal standard. A linear relationship was observed within the 0.2–20 ppm concentration range, in addition to a high correlation coefficient and 3% relative standard deviation.
Quantitation of lysinoalanine in dairy products by liquid chromatography–mass spectrometry with selective ion monitoring / Calabrese, MARIA GRAZIA; G., Mamone; S., Caira; Ferranti, Pasquale; Addeo, Francesco. - In: FOOD CHEMISTRY. - ISSN 0308-8146. - STAMPA. - 116:3(2009), pp. 799-805. [10.1016/j.foodchem.2009.03.041]
Quantitation of lysinoalanine in dairy products by liquid chromatography–mass spectrometry with selective ion monitoring
CALABRESE, MARIA GRAZIA;FERRANTI, PASQUALE;ADDEO, FRANCESCO
2009
Abstract
The unnatural amino acid lysinoalanine (LAL) has been identified in milk and cheese products by liquid chromatography mass spectrometry (LC/ESI/MS) with selective ion monitoring (SIM) of the 9-fluorenyl-methylchloro-formate (FMOC) derivative. LAL is not present in raw milk or derived from Mozzarella cheese; however, high amounts of LAL are found in calcium caseinate and milk powder. As expected, milk fortified with caseinate or whey protein powder produces cheese with higher LAL content. Our analytical procedure is based on the simultaneous detection of specific ion masses of the FMOC–LAL derivative and the N-ε-methyl-lysine internal standard. A linear relationship was observed within the 0.2–20 ppm concentration range, in addition to a high correlation coefficient and 3% relative standard deviation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
