Chemometric Validation of a High-Performance Liquid Chromatography Method to Detect Ochratoxin A in Green Coffee

Green coffee beans and their derivatives are employed in dietary supplements, pharmaceuticals, and cosmetic formulations owing to their antioxidant properties and secondary metabolites, which may play a role in preventing metabolic syndrome. Mycotoxins, particularly ochratoxin A (OTA), present a considerable contamination risk and are regulated under strict guidelines established by the European Union and various food safety authorities. This study validates a reverse-phase HPLC method with a fluorimetric detector for quantifying OTA in green coffee beans, following the ISO 17025:2018 standard. The OTA recovery rate (≥70%) met European Union (EU) Regulation No. 2023/2782. The calibration curve showed perfect linearity (r-value = 1). As EU legislation requires, the application field detected OTA levels between 3 µg/kg and 5 µg/kg. Precision (sr = 0.0073) and accuracy (±0.76 µg/kg) were statistically acceptable, with a 95% confidence level indicating reliable results.