This study presents an innovative methodology to analyze a blood sample from a healthy donor, providing a quantitative characterization of white blood cells (WBCs). It aims to evaluate the effectiveness of holographic quantitative phase imaging (QPI) flow cytometry (FC) in examining phase-contrast maps at the cellular level, thereby enabling the identification and classification of granulocyte types. Additionally, we demonstrate that an unsupervised method can differentiate granulocyte sub-types, i.e., neutrophils and eosinophils. The results instill strong confidence in the potential future use of QPI FC for liquid biopsies and/or for assessing the heterogeneity of WBCs and, more broadly, to facilitate label-free blood tests.
On the label-free analysis of white blood cells by holographic quantitative phase imaging flow cytometry / Giugliano, G., Pirone, D., Behal, J., Wang, Z., Cerbone, V., Mugnano, M., Licitra, F., Montella, A., Scalia, G., Capasso, M., Iolascon, A., Mari, S., Ferranti, F., Bianco, V., Maffettone, P.L., Memmolo, P., Miccio, L., Ferraro, P.. - In: JOURNAL OF THE OPTICAL SOCIETY OF AMERICA. A, OPTICS, IMAGE SCIENCE, AND VISION. - ISSN 1084-7529. - 41:12(2024). [10.1364/josaa.536841]
On the label-free analysis of white blood cells by holographic quantitative phase imaging flow cytometry
Pirone, Daniele;Behal, Jaromir;Wang, Zhe;Cerbone, Vincenza;Mugnano, Martina;Montella, Annalaura;Scalia, Giulia;Capasso, Mario;Iolascon, Achille;Maffettone, Pier Luca;Memmolo, Pasquale;Ferraro, Pietro
2024
Abstract
This study presents an innovative methodology to analyze a blood sample from a healthy donor, providing a quantitative characterization of white blood cells (WBCs). It aims to evaluate the effectiveness of holographic quantitative phase imaging (QPI) flow cytometry (FC) in examining phase-contrast maps at the cellular level, thereby enabling the identification and classification of granulocyte types. Additionally, we demonstrate that an unsupervised method can differentiate granulocyte sub-types, i.e., neutrophils and eosinophils. The results instill strong confidence in the potential future use of QPI FC for liquid biopsies and/or for assessing the heterogeneity of WBCs and, more broadly, to facilitate label-free blood tests.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
