: The discovery of plant-derived compounds that are able to combat antibiotic-resistant pathogens is an urgent demand. Over years, Centaurea hyalolepis attracted considerable attention because of its beneficial medical properties. Phytochemical analyses revealed that Centaurea plant species contain several metabolites, such as sesquiterpene lactones (STLs), essential oils, flavonoids, alkaloids, and lignans.The organic extract of C. hyalolepis plant, collected in Palestine, showed significant antimicrobial properties towards a panel of Gram-negative and Gram-positive bacterial strains when the Minimal Inhibitory Concentration (MIC) values were evaluated by broth microdilution assays. A bio-guided fractionation of the active extract via multiple steps of column and thin layer chromatography allowed us to obtain three main compounds. The isolated metabolites were identified as the STLs cnicin, 11β,13-dihydrosalonitenolide and salonitenolide by spectroscopic and spectrometric analyses. Cnicin conferred the strongest antimicrobial activity among the identified compounds. Moreover, the evaluation of its antibiofilm activity by biomass assays through crystal violet staining revealed almost 30% inhibition of biofilm formation in the case of A. baumannii ATCC 17878 strain. Furthermore, the quantification of carbohydrates and proteins present in the extracellular polymeric substance (EPS) revealed the ability of cnicin to significantly perturb biofilm structure. Based on these promising results, further investigations might open interesting perspectives to its applicability in biomedical field to counteract multidrug resistant infections.

Antimicrobial and antibiofilm activity of specialized metabolites isolated from Centaurea hyalolepis / Ismail, Shurooq; Masi, Marco; Gaglione, Rosa; Arciello, Angela; Cimmino, Alessio. - In: PEERJ. - ISSN 2167-8359. - 12:(2024). [10.7717/peerj.16973]

Antimicrobial and antibiofilm activity of specialized metabolites isolated from Centaurea hyalolepis

Ismail, Shurooq
Primo
Formal Analysis
;
Masi, Marco
Secondo
Writing – Review & Editing
;
Gaglione, Rosa
Writing – Review & Editing
;
Arciello, Angela
Penultimo
Project Administration
;
Cimmino, Alessio
Ultimo
Supervision
2024

Abstract

: The discovery of plant-derived compounds that are able to combat antibiotic-resistant pathogens is an urgent demand. Over years, Centaurea hyalolepis attracted considerable attention because of its beneficial medical properties. Phytochemical analyses revealed that Centaurea plant species contain several metabolites, such as sesquiterpene lactones (STLs), essential oils, flavonoids, alkaloids, and lignans.The organic extract of C. hyalolepis plant, collected in Palestine, showed significant antimicrobial properties towards a panel of Gram-negative and Gram-positive bacterial strains when the Minimal Inhibitory Concentration (MIC) values were evaluated by broth microdilution assays. A bio-guided fractionation of the active extract via multiple steps of column and thin layer chromatography allowed us to obtain three main compounds. The isolated metabolites were identified as the STLs cnicin, 11β,13-dihydrosalonitenolide and salonitenolide by spectroscopic and spectrometric analyses. Cnicin conferred the strongest antimicrobial activity among the identified compounds. Moreover, the evaluation of its antibiofilm activity by biomass assays through crystal violet staining revealed almost 30% inhibition of biofilm formation in the case of A. baumannii ATCC 17878 strain. Furthermore, the quantification of carbohydrates and proteins present in the extracellular polymeric substance (EPS) revealed the ability of cnicin to significantly perturb biofilm structure. Based on these promising results, further investigations might open interesting perspectives to its applicability in biomedical field to counteract multidrug resistant infections.
2024
Antimicrobial and antibiofilm activity of specialized metabolites isolated from Centaurea hyalolepis / Ismail, Shurooq; Masi, Marco; Gaglione, Rosa; Arciello, Angela; Cimmino, Alessio. - In: PEERJ. - ISSN 2167-8359. - 12:(2024). [10.7717/peerj.16973]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/976230
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