: Sensitisation to the lipid transfer protein Pru p 3 is associated with severe allergic reactions to peach, the proteins stability being thought to play a role in its allergenicity. Lipid binding increases susceptibility of Pru p 3 to digestion and so the impact of bile salts on the in vitro gastrointestinal digestibility of Pru p 3 was investigated and digestion products mapped by SDS-PAGE and mass spectrometry. Bile salts enhanced the digestibility of Pru p 3 resulting in an ensemble of around 100 peptides spanning the protein's sequence which were linked by disulphide bonds into structures of ~ 5-6 kDa. IgE binding studies with a serum panel from peach allergic subjects showed digestion reduced, but did not abolish, the IgE reactivity of Pru p 3. These data show the importance of including bile salts in vitro digestion systems and emphasise the need to profile of digestion in a manner that allows identification of immunologically relevant disulphide-linked peptide aggregates.

Bile salts enhance the susceptibility of the peach allergenic lipid transfer protein, Pru p 3, to in vitro gastrointestinal proteolysis / Wang, Kai; Gali-Moya, Judit; Ruano-Zaragoza, Maria; Cain, Kathleen; D’Auria, Giovanni; Daly, Matthew; Barran, Perdita; Crevel, René; Mills, E. N. Clare. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 13:1(2023). [10.1038/s41598-023-39599-0]

Bile salts enhance the susceptibility of the peach allergenic lipid transfer protein, Pru p 3, to in vitro gastrointestinal proteolysis

D’Auria, Giovanni
Investigation
;
2023

Abstract

: Sensitisation to the lipid transfer protein Pru p 3 is associated with severe allergic reactions to peach, the proteins stability being thought to play a role in its allergenicity. Lipid binding increases susceptibility of Pru p 3 to digestion and so the impact of bile salts on the in vitro gastrointestinal digestibility of Pru p 3 was investigated and digestion products mapped by SDS-PAGE and mass spectrometry. Bile salts enhanced the digestibility of Pru p 3 resulting in an ensemble of around 100 peptides spanning the protein's sequence which were linked by disulphide bonds into structures of ~ 5-6 kDa. IgE binding studies with a serum panel from peach allergic subjects showed digestion reduced, but did not abolish, the IgE reactivity of Pru p 3. These data show the importance of including bile salts in vitro digestion systems and emphasise the need to profile of digestion in a manner that allows identification of immunologically relevant disulphide-linked peptide aggregates.
2023
Bile salts enhance the susceptibility of the peach allergenic lipid transfer protein, Pru p 3, to in vitro gastrointestinal proteolysis / Wang, Kai; Gali-Moya, Judit; Ruano-Zaragoza, Maria; Cain, Kathleen; D’Auria, Giovanni; Daly, Matthew; Barran, Perdita; Crevel, René; Mills, E. N. Clare. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 13:1(2023). [10.1038/s41598-023-39599-0]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/957731
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