Microscopy Techniques

This book was born of close collaboration between Professor Antonio Giordano— a highly regarded pathologist—and Professor F.M. Sacerdoti, an engineer specializing in the realization of complex equipment and vision systems. We agreed that pathologists, biologists, medi- cal doctors, applied researchers, and others who work with imaging systems, such as microscopes, time-to-amplitude converter, etc., every day, would benefit from a deeper under- standing of how these systems work, what soft- ware can be used to process their information, why the image is so confusing, and so on. The first chapter is a basic guide to statistics, which is important for all working in the health field. We then provide an overview of the latest techniques in several medical fields, from pathol- ogy, microscopy, and cytometry to advanced microscopy techniques. Each chapter has been prepared by an expert in the field—someone who works with the spe- cific topic on a daily basis—to provide realistic insight into the techniques, their use, and specific lessons learned.