: Ribonucleoprotein (RNP) condensates are crucial for controlling RNA metabolism and splicing events in animal cells. We used spatial proteomics and transcriptomic to elucidate RNP interaction networks at the centrosome, the main microtubule-organizing center in animal cells. We found a number of cell-type specific centrosome-associated spliceosome interactions localized in subcellular structures involved in nuclear division and ciliogenesis. A component of the nuclear spliceosome BUD31 was validated as an interactor of the centriolar satellite protein OFD1. Analysis of normal and disease cohorts identified the cholangiocarcinoma as target of centrosome-associated spliceosome alterations. Multiplexed single-cell fluorescent microscopy for the centriole linker CEP250 and spliceosome components including BCAS2, BUD31, SRSF2 and DHX35 recapitulated bioinformatic predictions on the centrosome-associated spliceosome components tissue-type specific composition. Collectively, centrosomes and cilia act as anchor for cell-type specific spliceosome components, and provide a helpful reference for explore cytoplasmic condensates functions in defining cell identity and in the origin of rare diseases.

Single-cell proteo-genomic reveals a comprehensive map of centrosome-associated spliceosome components / Cerulo, Luigi; Pezzella, Nunziana; Caruso, Francesca Pia; Parente, Paola; Remo, Andrea; Giordano, Guido; Forte, Nicola; Busselez, Johan; Boschi, Federico; Galiè, Mirco; Franco, Brunella; Pancione, Massimo. - In: ISCIENCE. - ISSN 2589-0042. - 26:5(2023), p. 106602. [10.1016/j.isci.2023.106602]

Single-cell proteo-genomic reveals a comprehensive map of centrosome-associated spliceosome components

Pezzella, Nunziana;Franco, Brunella;
2023

Abstract

: Ribonucleoprotein (RNP) condensates are crucial for controlling RNA metabolism and splicing events in animal cells. We used spatial proteomics and transcriptomic to elucidate RNP interaction networks at the centrosome, the main microtubule-organizing center in animal cells. We found a number of cell-type specific centrosome-associated spliceosome interactions localized in subcellular structures involved in nuclear division and ciliogenesis. A component of the nuclear spliceosome BUD31 was validated as an interactor of the centriolar satellite protein OFD1. Analysis of normal and disease cohorts identified the cholangiocarcinoma as target of centrosome-associated spliceosome alterations. Multiplexed single-cell fluorescent microscopy for the centriole linker CEP250 and spliceosome components including BCAS2, BUD31, SRSF2 and DHX35 recapitulated bioinformatic predictions on the centrosome-associated spliceosome components tissue-type specific composition. Collectively, centrosomes and cilia act as anchor for cell-type specific spliceosome components, and provide a helpful reference for explore cytoplasmic condensates functions in defining cell identity and in the origin of rare diseases.
2023
Single-cell proteo-genomic reveals a comprehensive map of centrosome-associated spliceosome components / Cerulo, Luigi; Pezzella, Nunziana; Caruso, Francesca Pia; Parente, Paola; Remo, Andrea; Giordano, Guido; Forte, Nicola; Busselez, Johan; Boschi, Federico; Galiè, Mirco; Franco, Brunella; Pancione, Massimo. - In: ISCIENCE. - ISSN 2589-0042. - 26:5(2023), p. 106602. [10.1016/j.isci.2023.106602]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/925843
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact