Transmission electron microscopy and the use of glutaraldehyde-osmium fixation allow to distinguish norepinephrine from epinephrine granules in the adrenochromaffin cells, a difficult distinction with histochemical methods if both types of granules are present in the same cell. Here we describe all the steps necessary to process the adrenochromaffin tissue for the transmission electron microscopy; this protocol is suitable for any kind of adrenal tissue, and personally we used it in mammals, reptiles, and amphibians.

Transmission electron microscopy: a method for studying the adrenal chromaffin cells.

Anna Capaldo
Primo
;
Maria De Falco
Secondo
;
Luigi Rosati;Vincenza Laforgia
Ultimo
2023

Abstract

Transmission electron microscopy and the use of glutaraldehyde-osmium fixation allow to distinguish norepinephrine from epinephrine granules in the adrenochromaffin cells, a difficult distinction with histochemical methods if both types of granules are present in the same cell. Here we describe all the steps necessary to process the adrenochromaffin tissue for the transmission electron microscopy; this protocol is suitable for any kind of adrenal tissue, and personally we used it in mammals, reptiles, and amphibians.
978-1-0716-2670-2
978-1-0716-2671-9
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/906593
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