Configuration by osmotic eustress agents of the morphometric characteristics and the polyphenolic content of differently pigmented baby lettuce varieties in two successive harvests

Salinity eustress is one of the pre-harvest factors that can be used to improve the phyto-chemical profile and the quality attributes of horticultural species, and most of the studies are carried out using NaCl. In this work, we compared the effect of three mildly saline iso-osmotic nutrient solutions (NS) differing in the cation employed (either K, Na, or Ca) in baby lettuce cultivated in a floating system. Specifically, we analyzed the impact on key morphological traits and polyphenol composition in leaves in a completely randomized design experiment with the following experimental factors and levels: two differently pigmented lettuce varieties (green and full red), three NSs (20 mM KCl, 20 mM NaCl, 13.3 mM CaCl2, each with a final ionic concentration of 40 mM), and two successive harvests. The lettuce response to mild salinity was multifaceted and with a marked role for the cultivar factor and its interactions, as also indicated by multivariate analysis. The morphological response of baby lettuce to the saline solutions was predominantly affected by the osmolarity, and ion-specific alleviating or detrimental effects were not observed. The phytochemical analysis re-vealed significant interactions among all tested factors, with ion-specific differences over some of the detected phenolics. This is consistent with the high sensitivity of this class of bioactive compounds to environmental factors. This work indicated that certain combinations of the experimental factors tested can be exploited to improve the biochemical profile and possibly the nutraceutical quality of baby lettuce in a floating system.