The emerging concern about the increase of antibiotic resistance has encouraged research efforts to develop effective alternatives to counteract bacterial infections. Herein, we studied a new perspective to therapeutic treatment against Staphylococcus pseudintermedius, an opportunistic pathogen documented as the major cause of skin, ear, and post-operative bacterial infections in dogs and cats. Antimicrobial activity of secondary metabolites produced by selected microbial strains belonging to Trichoderma, Talaromyces, Clonostachys and Coniothyrium fungal genera has been tested against S. pseudintermedius. Several extracts, particularly those obtained from Trichoderma harzianum E45 and ET45, showed a significant antimicrobial activity towards S. pseudintermedius methicillin-resistant (MRSP) and methicillin-susceptible (MSSP) strains. Bioassay-guided fractionation of E45 and ET45 extracts allowed to isolate harzianic acid as the major compound responsible for biological activities (e.g. antimicrobial, antibiofilm formation and biofilm disaggregation).

Antimicrobial activity of harzianic acid against Staphylococcus pseudintermedius

Anna De Filippis
Membro del Collaboration Group
;
Francesca Paola Nocera
Membro del Collaboration Group
;
Simona Tafuri
Membro del Collaboration Group
;
Francesca Ciani
Membro del Collaboration Group
;
Ernesto Comite
Membro del Collaboration Group
;
Assunta Bottiglieri
Membro del Collaboration Group
;
Laura Gioia
Membro del Collaboration Group
;
Matteo Lorito
Membro del Collaboration Group
;
Sheridan Lois Woo
Membro del Collaboration Group
;
Francesco Vinale
Investigation
;
Luisa De Martino
Membro del Collaboration Group
2021

Abstract

The emerging concern about the increase of antibiotic resistance has encouraged research efforts to develop effective alternatives to counteract bacterial infections. Herein, we studied a new perspective to therapeutic treatment against Staphylococcus pseudintermedius, an opportunistic pathogen documented as the major cause of skin, ear, and post-operative bacterial infections in dogs and cats. Antimicrobial activity of secondary metabolites produced by selected microbial strains belonging to Trichoderma, Talaromyces, Clonostachys and Coniothyrium fungal genera has been tested against S. pseudintermedius. Several extracts, particularly those obtained from Trichoderma harzianum E45 and ET45, showed a significant antimicrobial activity towards S. pseudintermedius methicillin-resistant (MRSP) and methicillin-susceptible (MSSP) strains. Bioassay-guided fractionation of E45 and ET45 extracts allowed to isolate harzianic acid as the major compound responsible for biological activities (e.g. antimicrobial, antibiofilm formation and biofilm disaggregation).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/822065
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