We report an innovative fiber optic biosensor based on Long Period Gratings (LPGs) working in reflection configuration for real time monitoring of human Thyroglobulin (Tg), a protein marker of differentiated thyroid cancer. A standard LPG is first transformed in a practical probe working in reflection mode, and then it is coated with a single layer of atactic polystyrene (aPS) in order to increase its surrounding refractive index sensitivity and to provide, at the same time, the desired interfacial properties for a stable anti-Tg antibody. The functionalized reflection-type LPG biosensor clearly demonstrates the effectiveness and sensitivity of the developed biosensing platform, allowing the real time and label-free detection of Tg in the needle washouts of fine-needle aspiration biopsies, at concentrations useful for pre- and post-operative assessment of the biomarker levels. Analyte recognition and capture were confirmed with a parallel on fiber ELISA-like assay using, in pilot test s, the biotinylated protein and HRP-labeled streptavidin for its detection. Dose-dependent experiments showed that the detection is linearly dependent on concentration within the range between 0 and 4 ng/mL, while antibody saturation occurs for higher protein levels.

High Sensitive Long Period Fiber Grating Biosensor for Cancer Biomarker Detection / Cusano, Andrea; Cutolo, Antonello; Santorelli, Flavio; Macchia, Paolo Emidio; Colao, Annamaria; Nettore, Immacolata Cristina; Giordano, Michele; Zuppolini, Simona; Diodato, Laura; Borriello, Anna; Ruvo, Menotti; Sandomenico, Annamaria; Boniello, Alessandra; Vaiano, Patrizio; Severino, Renato; Consales, Marco; Quero, Giuseppe. - 5:(2016), pp. 561-569. [10.5220/0005846705610569]

High Sensitive Long Period Fiber Grating Biosensor for Cancer Biomarker Detection

Cutolo, Antonello
;
Macchia, Paolo Emidio;Colao, Annamaria;Nettore, Immacolata Cristina;Boniello, Alessandra;
2016

Abstract

We report an innovative fiber optic biosensor based on Long Period Gratings (LPGs) working in reflection configuration for real time monitoring of human Thyroglobulin (Tg), a protein marker of differentiated thyroid cancer. A standard LPG is first transformed in a practical probe working in reflection mode, and then it is coated with a single layer of atactic polystyrene (aPS) in order to increase its surrounding refractive index sensitivity and to provide, at the same time, the desired interfacial properties for a stable anti-Tg antibody. The functionalized reflection-type LPG biosensor clearly demonstrates the effectiveness and sensitivity of the developed biosensing platform, allowing the real time and label-free detection of Tg in the needle washouts of fine-needle aspiration biopsies, at concentrations useful for pre- and post-operative assessment of the biomarker levels. Analyte recognition and capture were confirmed with a parallel on fiber ELISA-like assay using, in pilot test s, the biotinylated protein and HRP-labeled streptavidin for its detection. Dose-dependent experiments showed that the detection is linearly dependent on concentration within the range between 0 and 4 ng/mL, while antibody saturation occurs for higher protein levels.
2016
978-989-758-170-0
High Sensitive Long Period Fiber Grating Biosensor for Cancer Biomarker Detection / Cusano, Andrea; Cutolo, Antonello; Santorelli, Flavio; Macchia, Paolo Emidio; Colao, Annamaria; Nettore, Immacolata Cristina; Giordano, Michele; Zuppolini, Simona; Diodato, Laura; Borriello, Anna; Ruvo, Menotti; Sandomenico, Annamaria; Boniello, Alessandra; Vaiano, Patrizio; Severino, Renato; Consales, Marco; Quero, Giuseppe. - 5:(2016), pp. 561-569. [10.5220/0005846705610569]
File in questo prodotto:
File Dimensione Formato  
SmartMedDev_2016_7.pdf

accesso aperto

Tipologia: Documento in Post-print
Licenza: Dominio pubblico
Dimensione 740.03 kB
Formato Adobe PDF
740.03 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/810106
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? ND
social impact