Paralytic shellfish poisoning toxins (PST) are a group of neurotoxins produced by dinoflagellate belonging to genera Alexandrium and Gymnodinium. The accumulation of these toxins in seafood represent a major concern to humans since following ingestion of contaminated seafood human poisonings with neurological symptomatology may occur. Consequently, official control monitoring of these toxins is carried out in Europe. Tetrodotoxin (TTX) is a neurotoxin firstly detected in fish parrotfish and triggerfish; it has been recently found in European seafood (Spain, Portugal, England, Greece, etc). Due to its chemical similarity with PST, TTX may be co-extracted with any of the extraction procedures currently used for PST. Thus, a method that allows the combined analysis of PST and TTX is highly desirable. The aim of this work was to set up a method based on hydrophilic interaction liquid chromatography coupled to high resolution multiple stage mass spectrometry (HILIC-HRMSn) for the combined determination of PST and TTX in different matrices. The method has been successfully applied to the analysis of real samples, including marine phytoplankton, algal cultures of Alexandrium minutum, A. pacificum and mussel extracts collected in the frame of a monitoring study of these toxins in the Mediterranean Sea carried out from 2012 to nowadays. Different HILIC columns were tested and results obtained by HILIC-HRMSn on an LTQ Orbitrap XL were compared with those obtained by HILIC-MS/MS on a triple quadrupole MS.

LC-HRMSn versus LC-MS/MS of Paralytic Shellfish Poisoning toxins and tetrodotoxin in microalgae and seafood

Luciana Tartaglione
;
Carmela Dell’Aversano
2017

Abstract

Paralytic shellfish poisoning toxins (PST) are a group of neurotoxins produced by dinoflagellate belonging to genera Alexandrium and Gymnodinium. The accumulation of these toxins in seafood represent a major concern to humans since following ingestion of contaminated seafood human poisonings with neurological symptomatology may occur. Consequently, official control monitoring of these toxins is carried out in Europe. Tetrodotoxin (TTX) is a neurotoxin firstly detected in fish parrotfish and triggerfish; it has been recently found in European seafood (Spain, Portugal, England, Greece, etc). Due to its chemical similarity with PST, TTX may be co-extracted with any of the extraction procedures currently used for PST. Thus, a method that allows the combined analysis of PST and TTX is highly desirable. The aim of this work was to set up a method based on hydrophilic interaction liquid chromatography coupled to high resolution multiple stage mass spectrometry (HILIC-HRMSn) for the combined determination of PST and TTX in different matrices. The method has been successfully applied to the analysis of real samples, including marine phytoplankton, algal cultures of Alexandrium minutum, A. pacificum and mussel extracts collected in the frame of a monitoring study of these toxins in the Mediterranean Sea carried out from 2012 to nowadays. Different HILIC columns were tested and results obtained by HILIC-HRMSn on an LTQ Orbitrap XL were compared with those obtained by HILIC-MS/MS on a triple quadrupole MS.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11588/737494
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