Recent developments in applied research have sought to incorporate novel operative protocol for agriculture-based restoration of contaminated agricultural soils, multi-functionality and landscape identity in the NIPS (National Interest Priority Site). Topics of LIFE 11 ENV/IT/ 275 ECOMERED project include, in C2c sub-action, the bio-monitoring of oxidative damage and characterization of reproductive health status of selected vertebrate and invertebrate species. The gonadal tissue, with abundance of highly unsaturated fatty acids, high rates of cell division, and variety of testis enzymes results very vulnerable to the overexpression of reactive oxygen species (ROS). Our approach highlights the importance to assess, before and after remediation in one testis gonadectomy of i.e. barcoded lizards, the oxidative damage starting from the level of ROS using ESR spectroscopy and to analyze the steroid receptors gene regulation of antioxidant stress enzymes. It also deals with of the advantages to follow testis histological variation and the gene expression of an antioxidant under steroid control, the major selenoprotein expressed by germ cells in the testis, the phospholipid hydroperoxide glutathione peroxidase (PHGPx/GPx4). GPx4, in particular, represents the pivotal link between sperm quality, species preservation and soil bioremediation indicator establishing a connection between the disrupters effect in the organism and its environment.
Lizard spermatogenesis as soil remediation biosensor: LIFE 11 ENV/IT/275 ECOMERED project, C2c sub-action (LECTURE) / Guerriero, Giulia. - (2014). (Intervento presentato al convegno The 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology tenutosi a Okazaki Conference Center - National Institute for Basic Biology, Okazaki, Japan nel Nov. 7 - 9, 2014).
Lizard spermatogenesis as soil remediation biosensor: LIFE 11 ENV/IT/275 ECOMERED project, C2c sub-action (LECTURE)
GUERRIERO, GIULIA
2014
Abstract
Recent developments in applied research have sought to incorporate novel operative protocol for agriculture-based restoration of contaminated agricultural soils, multi-functionality and landscape identity in the NIPS (National Interest Priority Site). Topics of LIFE 11 ENV/IT/ 275 ECOMERED project include, in C2c sub-action, the bio-monitoring of oxidative damage and characterization of reproductive health status of selected vertebrate and invertebrate species. The gonadal tissue, with abundance of highly unsaturated fatty acids, high rates of cell division, and variety of testis enzymes results very vulnerable to the overexpression of reactive oxygen species (ROS). Our approach highlights the importance to assess, before and after remediation in one testis gonadectomy of i.e. barcoded lizards, the oxidative damage starting from the level of ROS using ESR spectroscopy and to analyze the steroid receptors gene regulation of antioxidant stress enzymes. It also deals with of the advantages to follow testis histological variation and the gene expression of an antioxidant under steroid control, the major selenoprotein expressed by germ cells in the testis, the phospholipid hydroperoxide glutathione peroxidase (PHGPx/GPx4). GPx4, in particular, represents the pivotal link between sperm quality, species preservation and soil bioremediation indicator establishing a connection between the disrupters effect in the organism and its environment.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
