In C. capitata sex determination is controlled by a Y-linked male determining factor (M-factor) which influences, either directly or indirectly, the state of activity of the female determining master gene Cctraep (Cctra epigenetic) at 8-10 hours after egg laying. We have developed a Medfly transgenic sexing strain which produces male-only progeny (95% efficiency) by transgene-mediated RNAi against the female determiner Cctraep gene. C. capitata XX males are fully fertile and could be successfully mated with XX females thus leading to the production of female-only (XX) progeny. Starting from two polyA+ RNA preparations, extracted from XX-only and XYXX medfly embryos at 8-10 hours from oviposition, we have defined two de novo transcriptome sets consisting respectively of 42614 (XYXX, N50=2158) and 39255 (XX, N50=2047) assembled transcripts. In this talk we will outline the assembly testing pipeline, developed with a combination of different on-line and stand alone tools, and preliminary data on the in silico subtraction approach for the identification of male-biased genes/male-determining genes.

Medfly early embryonic sexed transcriptomes: in silico approaches to identify sex-biased/male determining genes / Salvemini, Marco; D'Amato, Rocco; Petrella, Valeria; Nagaraju, J; Polito, Catello; Saccone, Giuseppe. - (2013). (Intervento presentato al convegno Workshop “Nothiger Meeting” del Gruppo Europeo “Sex Determination in insects” tenutosi a Gif-sur-Yvette, Parigi, Francia nel 12-14 Giugno 2013).

Medfly early embryonic sexed transcriptomes: in silico approaches to identify sex-biased/male determining genes.

SALVEMINI, MARCO;D'AMATO, ROCCO;PETRELLA, VALERIA;POLITO, CATELLO;SACCONE, GIUSEPPE
2013

Abstract

In C. capitata sex determination is controlled by a Y-linked male determining factor (M-factor) which influences, either directly or indirectly, the state of activity of the female determining master gene Cctraep (Cctra epigenetic) at 8-10 hours after egg laying. We have developed a Medfly transgenic sexing strain which produces male-only progeny (95% efficiency) by transgene-mediated RNAi against the female determiner Cctraep gene. C. capitata XX males are fully fertile and could be successfully mated with XX females thus leading to the production of female-only (XX) progeny. Starting from two polyA+ RNA preparations, extracted from XX-only and XYXX medfly embryos at 8-10 hours from oviposition, we have defined two de novo transcriptome sets consisting respectively of 42614 (XYXX, N50=2158) and 39255 (XX, N50=2047) assembled transcripts. In this talk we will outline the assembly testing pipeline, developed with a combination of different on-line and stand alone tools, and preliminary data on the in silico subtraction approach for the identification of male-biased genes/male-determining genes.
2013
Medfly early embryonic sexed transcriptomes: in silico approaches to identify sex-biased/male determining genes / Salvemini, Marco; D'Amato, Rocco; Petrella, Valeria; Nagaraju, J; Polito, Catello; Saccone, Giuseppe. - (2013). (Intervento presentato al convegno Workshop “Nothiger Meeting” del Gruppo Europeo “Sex Determination in insects” tenutosi a Gif-sur-Yvette, Parigi, Francia nel 12-14 Giugno 2013).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/593786
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