Background: The present study investigates immunological cross-reactivity between Par o 1, the major pollen allergen of Parietaria, and the VP4 protein of rotavirus, a microorganism that is world-wide the main etiological agent of gastroenteritis in children. Methods: IgG and IgE cross-reactivity was assessed by direct binding and competitive inhibition assays (ELISA and DARIA), using recombinant VP4 from rhesus infectious rotavirus (RR), synthetic peptides and Par o 1-specific antibodies affinity purified from pooled and individual human sera. Results: Antibodies specifically binding Par o 1, affinity purified from the sera of 35 individuals with skin test positivity to Parietaria and from 14 pools, were extensively cross-reactive with RRVP4. Cross-reactive binding was specifically inhibited by synthetic peptides derived from the C-terminal sequences of the VP4 proteins from human and rhesus infectious rotavirus. Conclusions: This study reports the first evidence of cross-reactivity between an allergen and a viral antigen.
Cross-reactivity between the major Parietaria allergen and rotavirus VP4 protein / C., di Somma; L., Fiore; A., Di Lonardo; B., Ridolfi; Garzillo, Carmine; A., Chersi; C., Buono; T., Menna; A., Ruffilli. - In: ALLERGY. - ISSN 0105-4538. - 58:(2003), pp. 503-510. [10.1034/j.1398-9995.2003.00176.x]
Cross-reactivity between the major Parietaria allergen and rotavirus VP4 protein
GARZILLO, CARMINE;
2003
Abstract
Background: The present study investigates immunological cross-reactivity between Par o 1, the major pollen allergen of Parietaria, and the VP4 protein of rotavirus, a microorganism that is world-wide the main etiological agent of gastroenteritis in children. Methods: IgG and IgE cross-reactivity was assessed by direct binding and competitive inhibition assays (ELISA and DARIA), using recombinant VP4 from rhesus infectious rotavirus (RR), synthetic peptides and Par o 1-specific antibodies affinity purified from pooled and individual human sera. Results: Antibodies specifically binding Par o 1, affinity purified from the sera of 35 individuals with skin test positivity to Parietaria and from 14 pools, were extensively cross-reactive with RRVP4. Cross-reactive binding was specifically inhibited by synthetic peptides derived from the C-terminal sequences of the VP4 proteins from human and rhesus infectious rotavirus. Conclusions: This study reports the first evidence of cross-reactivity between an allergen and a viral antigen.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
