In a search for open reading frames in several known polynucleotide sequences of structural genes, we found that the anti-sense strand of the β and ε human, α and β rabbit globin DNA fragments contain translatable sequences even longer than the coding sequences of the globin strand. A more detailed analysis of the human β and ε sequences reveals that (a) Translatable sequences use the same frame as the globin coding strand, with the opposite polarity (b) None of the many β globin variants either introduces a termination codon or removes the putative AUG initiation codon in the anti-sense strand (c) Leader sequences identical to those found in the 5’ non-coding region of globin mRNA, are found upstream the AUG codon (d) Sequences complementary to both introns, present on both β and ε globin genes, contain termination codons in all reading frames, as in the case of the intron sequences themselves, and splicing rules are respected. We postulate the existence of protein(s) coded by the complementary strand of β and ε human globin translated strand and that these two hypothetical genes also contain untranslated intron sequences.
Polynucleotide Analysis of Human Globin Variants / A., Cascino; M., Cipollaro; V., Scarlato; Mastrocinque, Giuseppe. - (1980). (Intervento presentato al convegno XVIII Congr. of Intern. Soc. of Hematology, Montreal 1980 tenutosi a Montreal, Québec nel Agosto 1980).
Polynucleotide Analysis of Human Globin Variants
MASTROCINQUE, GIUSEPPE
1980
Abstract
In a search for open reading frames in several known polynucleotide sequences of structural genes, we found that the anti-sense strand of the β and ε human, α and β rabbit globin DNA fragments contain translatable sequences even longer than the coding sequences of the globin strand. A more detailed analysis of the human β and ε sequences reveals that (a) Translatable sequences use the same frame as the globin coding strand, with the opposite polarity (b) None of the many β globin variants either introduces a termination codon or removes the putative AUG initiation codon in the anti-sense strand (c) Leader sequences identical to those found in the 5’ non-coding region of globin mRNA, are found upstream the AUG codon (d) Sequences complementary to both introns, present on both β and ε globin genes, contain termination codons in all reading frames, as in the case of the intron sequences themselves, and splicing rules are respected. We postulate the existence of protein(s) coded by the complementary strand of β and ε human globin translated strand and that these two hypothetical genes also contain untranslated intron sequences.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
