Enniatins (ENs) are antibiotic compounds of hexadepsipeptidic structure produced by several strains of Fusarium spp. The ENs A, A(1), B, B-1 were purified from extracts of Fusarium tricinctum grown on a solid medium of corn, by a low pressure liquid chromatography (LPLC) on reverse phase of Amberlite XAD-7 followed by semipreparative LC. The purity and the structure of the isolated compounds were confirmed by LC-MS/MS. The technique of the purification of the fungal extract enabled complete separation of the ENs A, A(1), B, B-1 with a mean purity of 97% for all the compounds. The cytoxicity of the ENs was tested in the cell lines of human origin (epithelial colorectal adenocarcinoma cells, Caco-2) by MTT assays. Only EN A(1) and B-1 evoked toxicity at the tested concentrations. The inhibitory concentration (IC50) for EN A(1) on Caco-2 cells was 12.3 mu M, whereas the IC50 produced by the EN B-1 was 19.5 mu M. This study indicates that ENs, fungal metabolites that are commonly found in corn and in general in product composed by corn, may have a toxic potential for human health. (C) 2010 Elsevier Ltd. All rights reserved.
Isolation and purification of enniatins A, A(1), B, B-1, produced by Fusarium tricinctum in solid culture, and cytotoxicity effects on Caco-2 cells / G., Meca; M. J., Ruiz; J. M., Soriano; Ritieni, Alberto; A., Moretti; G., Font; J., Manes. - In: TOXICON. - ISSN 0041-0101. - STAMPA. - 56:(2010), pp. 418-424. [10.1016/j.toxicon.2010.04.008]
Isolation and purification of enniatins A, A(1), B, B-1, produced by Fusarium tricinctum in solid culture, and cytotoxicity effects on Caco-2 cells
RITIENI, ALBERTO;
2010
Abstract
Enniatins (ENs) are antibiotic compounds of hexadepsipeptidic structure produced by several strains of Fusarium spp. The ENs A, A(1), B, B-1 were purified from extracts of Fusarium tricinctum grown on a solid medium of corn, by a low pressure liquid chromatography (LPLC) on reverse phase of Amberlite XAD-7 followed by semipreparative LC. The purity and the structure of the isolated compounds were confirmed by LC-MS/MS. The technique of the purification of the fungal extract enabled complete separation of the ENs A, A(1), B, B-1 with a mean purity of 97% for all the compounds. The cytoxicity of the ENs was tested in the cell lines of human origin (epithelial colorectal adenocarcinoma cells, Caco-2) by MTT assays. Only EN A(1) and B-1 evoked toxicity at the tested concentrations. The inhibitory concentration (IC50) for EN A(1) on Caco-2 cells was 12.3 mu M, whereas the IC50 produced by the EN B-1 was 19.5 mu M. This study indicates that ENs, fungal metabolites that are commonly found in corn and in general in product composed by corn, may have a toxic potential for human health. (C) 2010 Elsevier Ltd. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
