A calorimetric study is reported on the interaction between allosteric bovine seminal ribonuclease and cytidine-3'-phosphate. The results are compared with those reported for the interaction of pancreatic ribonuclease A and the same nucleoside. The analysis of the data provides evidence the the binding sites of seminal ribonuclease for cytidine-3'-phosphate are nor equivalent, in agreement with previous studies. A model with two sites with different affinities toward the nucleotide, the site with higher affinity resembling the binding site of pancreatic ribonuclease, is proposed.
A Calorimetric Approach to the Study of the Interactions of Cytidine-3'-Phosphate with Bovine Seminal Ribonuclease / Ambrosino, R.; Barone, Guido; Castronuovo, Giuseppina; Cultrera, O.; DI DONATO, Alberto; Elia, Vittorio. - In: BIOPOLYMERS. - ISSN 0006-3525. - STAMPA. - 28:(1989), pp. 1403-1411.
A Calorimetric Approach to the Study of the Interactions of Cytidine-3'-Phosphate with Bovine Seminal Ribonuclease
BARONE, GUIDO;CASTRONUOVO, GIUSEPPINA;DI DONATO, ALBERTO;ELIA, VITTORIO
1989
Abstract
A calorimetric study is reported on the interaction between allosteric bovine seminal ribonuclease and cytidine-3'-phosphate. The results are compared with those reported for the interaction of pancreatic ribonuclease A and the same nucleoside. The analysis of the data provides evidence the the binding sites of seminal ribonuclease for cytidine-3'-phosphate are nor equivalent, in agreement with previous studies. A model with two sites with different affinities toward the nucleotide, the site with higher affinity resembling the binding site of pancreatic ribonuclease, is proposed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
