A calorimetric study is reported on the interaction between allosteric bovine seminal ribonuclease and cytidine-3'-phosphate. The results are compared with those reported for the interaction of pancreatic ribonuclease A and the same nucleoside. The analysis of the data provides evidence the the binding sites of seminal ribonuclease for cytidine-3'-phosphate are nor equivalent, in agreement with previous studies. A model with two sites with different affinities toward the nucleotide, the site with higher affinity resembling the binding site of pancreatic ribonuclease, is proposed.

A Calorimetric Approach to the Study of the Interactions of Cytidine-3'-Phosphate with Bovine Seminal Ribonuclease

BARONE, GUIDO;CASTRONUOVO, GIUSEPPINA;DI DONATO, ALBERTO;ELIA, VITTORIO
1989

Abstract

A calorimetric study is reported on the interaction between allosteric bovine seminal ribonuclease and cytidine-3'-phosphate. The results are compared with those reported for the interaction of pancreatic ribonuclease A and the same nucleoside. The analysis of the data provides evidence the the binding sites of seminal ribonuclease for cytidine-3'-phosphate are nor equivalent, in agreement with previous studies. A model with two sites with different affinities toward the nucleotide, the site with higher affinity resembling the binding site of pancreatic ribonuclease, is proposed.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/456127
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