This study reports on the determination of toxin profile of mussels (Mytilus galloprovincialis) collected in November-December 2003 along the Emilia Romagna coasts (Italy) when a high concentration of Alexandrium ostenfeldii cells was detected in sea water. Detailed liquid chromatography-mass spectrometry (LC-MS) analyses were performed on the crude extracts in both selected ion monitoring (SIM) and multiple reaction monitoring (MRM) modes. They revealed that M. galloprovincialis had accumulated the three major spirolides produced by the alga, namely 13-desMethyl spirolide C, 13,19-didesMethyl spirolide C and 27-hydroxy-13,19-didesMethyl spirolide C, which fully accounted for toxicity of lipophilic extracts shown in mouse bioassay. Interestingly, yessotoxin (YTX) and its analogues were still present in mussel polar extracts but YTX itself was not the major toxin contained in mussels. The presence of pectenotoxin-2 seco acid (PTX-2sa) and its putative epimer was also assessed.
Complex toxin profile of Mytilus galloprovincialis from the Adriatic sea revealed by LC–MS / Tartaglione, Luciana; Ciminiello, Patrizia; Dell'Aversano, Carmela; Ernesto, Fattorusso; Forino, Martino; Lari, Boschetti; Silva, Rubini; Monica, Cangini; Silvia, Pigozzi; Roberto, Poletti. - (2009), pp. P36-P36. (Intervento presentato al convegno VIII Convegno Nazionale Giornate di Chimica delle Sostanze Naturali tenutosi a Forte dei Marmi (Lu), Italia nel 22-25 Maggio 2009).
Complex toxin profile of Mytilus galloprovincialis from the Adriatic sea revealed by LC–MS.
TARTAGLIONE, LUCIANA;CIMINIELLO, PATRIZIA;DELL'AVERSANO, CARMELA;FORINO, MARTINO;
2009
Abstract
This study reports on the determination of toxin profile of mussels (Mytilus galloprovincialis) collected in November-December 2003 along the Emilia Romagna coasts (Italy) when a high concentration of Alexandrium ostenfeldii cells was detected in sea water. Detailed liquid chromatography-mass spectrometry (LC-MS) analyses were performed on the crude extracts in both selected ion monitoring (SIM) and multiple reaction monitoring (MRM) modes. They revealed that M. galloprovincialis had accumulated the three major spirolides produced by the alga, namely 13-desMethyl spirolide C, 13,19-didesMethyl spirolide C and 27-hydroxy-13,19-didesMethyl spirolide C, which fully accounted for toxicity of lipophilic extracts shown in mouse bioassay. Interestingly, yessotoxin (YTX) and its analogues were still present in mussel polar extracts but YTX itself was not the major toxin contained in mussels. The presence of pectenotoxin-2 seco acid (PTX-2sa) and its putative epimer was also assessed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
