Recently, four RNases from zebrafish (Danio rerio) have been identified and studied (ZF-RNase-1, -2, -3 and -4) [1-3]. Kazakou et al. [2], after an extensive survey of zebrafish DNA sequences, which indicated widespread polymorphism among the known ZF-RNase homologues, determined the X-ray structure of ZF-RNase-1 and -3 at basic pH. They also describe a polymorphic variant of the gene encoding ZF-RNase-2. More recently, we have proved that this variant is indeed a cell product by itself [4]. Here we report the X-ray structure of ZF-RNase-5 and that of ZF-RNase-1 at acidic and neutral pH. The structures have been directly compared with those described by Kazakou et al. [2] and with the structures of ZF-RNase-2 and -4, which have been obtained by homology modelling (Fig.1). The results have allowed to explain some differences in the catalytic activity of these proteins [4].

Structure-catalytic activity relationship in zebrafish RNases / F., Coscia; RUSSO KRAUSS, Irene; Merlino, Antonello; Pizzo, Eliodoro; G., D’Alessio; Mazzarella, Lelio; Sica, Filomena. - STAMPA. - (2010), pp. 53-53. (Intervento presentato al convegno 8th International RNase Congress tenutosi a Napoli nel 20-22 ottobre 2010).

Structure-catalytic activity relationship in zebrafish RNases

RUSSO KRAUSS, IRENE;MERLINO, ANTONELLO;PIZZO, ELIODORO;MAZZARELLA, LELIO;SICA, FILOMENA
2010

Abstract

Recently, four RNases from zebrafish (Danio rerio) have been identified and studied (ZF-RNase-1, -2, -3 and -4) [1-3]. Kazakou et al. [2], after an extensive survey of zebrafish DNA sequences, which indicated widespread polymorphism among the known ZF-RNase homologues, determined the X-ray structure of ZF-RNase-1 and -3 at basic pH. They also describe a polymorphic variant of the gene encoding ZF-RNase-2. More recently, we have proved that this variant is indeed a cell product by itself [4]. Here we report the X-ray structure of ZF-RNase-5 and that of ZF-RNase-1 at acidic and neutral pH. The structures have been directly compared with those described by Kazakou et al. [2] and with the structures of ZF-RNase-2 and -4, which have been obtained by homology modelling (Fig.1). The results have allowed to explain some differences in the catalytic activity of these proteins [4].
2010
Structure-catalytic activity relationship in zebrafish RNases / F., Coscia; RUSSO KRAUSS, Irene; Merlino, Antonello; Pizzo, Eliodoro; G., D’Alessio; Mazzarella, Lelio; Sica, Filomena. - STAMPA. - (2010), pp. 53-53. (Intervento presentato al convegno 8th International RNase Congress tenutosi a Napoli nel 20-22 ottobre 2010).
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/374807
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