Understanding molecular mechanisms underlying tolerance to water deficit and cross-interaction between response to reduced water availability and fruit quality enhancement may help breeders to develop superior tomato genotypes for sustainable cropping systems. Water deficit tolerance is a quantitative trait and previously in our laboratories a QTL (Quantitative Trail Loci) for this trait was identified in the IL9-2-5, a Solanum pennellii introgression line. The aim of this research was to identify gene networks controlling fruit quality under water deficiency in a susceptible (M82) and a tolerant tomato genotype (IL 9-2-5). Plants were grown according to a split-plot design with three replicates in semi-controlled conditions. Three different levels of water restitution were applied. As water restitution decreased, IL9-2-5 showed a lower yield loss than M82. Also, IL9-2-5 performed higher ascorbate concentration in red-ripe fruit at lower water restitution volume. A comparative transcriptomic analysis of red-ripe fruit from IL9-2-5 and M82 at different water treatments was carried out on the Combimatrix TomatArray1.0. It allowed the identification of 160 differentially expressed transcripts between genotypes and 241 among treatments. A model network describing the interactions between the plant response to water deficit and the increased fruit quality was developed. It included genes involved in stress perception and transduction, ethylene biosynthesis and antioxidant metabolism. Currently, candidate transcripts are being validated by qPCR approach. Functional characterization of candidate genes will prove their involvement in fruit quality control and provide additional genetic means for tomato quality enhancement in high sustainable cropping systems.

Genetic control of fruit quality traits in tomato plants under water deficiency

DI MATTEO, ANTONIO;VASCO, MARIA;BARONE, AMALIA
2010

Abstract

Understanding molecular mechanisms underlying tolerance to water deficit and cross-interaction between response to reduced water availability and fruit quality enhancement may help breeders to develop superior tomato genotypes for sustainable cropping systems. Water deficit tolerance is a quantitative trait and previously in our laboratories a QTL (Quantitative Trail Loci) for this trait was identified in the IL9-2-5, a Solanum pennellii introgression line. The aim of this research was to identify gene networks controlling fruit quality under water deficiency in a susceptible (M82) and a tolerant tomato genotype (IL 9-2-5). Plants were grown according to a split-plot design with three replicates in semi-controlled conditions. Three different levels of water restitution were applied. As water restitution decreased, IL9-2-5 showed a lower yield loss than M82. Also, IL9-2-5 performed higher ascorbate concentration in red-ripe fruit at lower water restitution volume. A comparative transcriptomic analysis of red-ripe fruit from IL9-2-5 and M82 at different water treatments was carried out on the Combimatrix TomatArray1.0. It allowed the identification of 160 differentially expressed transcripts between genotypes and 241 among treatments. A model network describing the interactions between the plant response to water deficit and the increased fruit quality was developed. It included genes involved in stress perception and transduction, ethylene biosynthesis and antioxidant metabolism. Currently, candidate transcripts are being validated by qPCR approach. Functional characterization of candidate genes will prove their involvement in fruit quality control and provide additional genetic means for tomato quality enhancement in high sustainable cropping systems.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11588/370907
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