D-Aspartic Acid in the Nervous System of a Mollusc: An Immunohistochemical Protocol

D-Aspartic acid (D-Asp) was first detected by biochemical methods in the nervous tissue of cephalopod mollusc. Subsequently, this endogenous amino acid has been described in various tissues of the representatives of most vertebrate and invertebrate groups. Recently, D-Asp was described in the nervous system of Aplysia limacina (opisthobranch gasteropod mollusc) and its possible role in neurotransmission was suggested. Here we describe an immunohistochemical protocol to demonstrate the intracellular distribution of D-Asp in the cerebral ganglia of A. limacina.