Fast atom bombardment mass spectrometry has been applied to the localization of phosphorylation sites in buffalo beta-casein. Two complementary strategies of identification are described. Phosphorylated residues in the tryptic peptide Tp 1 have been assigned by measuring the masses of peptide fragments obtained by enzymatic degradations. The phosphoserine residue in peptide Tp 2 has been identified by determining the intact molecular weight and confirmed by partial sequence information. This rapid and sensitive procedure appears of a great interest in structural studies of a wide range of post-translational modifications in proteins.

Assignment of phosphorylation sites in buffalo beta-casein by fast atom bombardment mass spectrometry / Petrilli, Pasquale; Pucci, Pietro; Morris, Hr; Addeo, Francesco. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 140:1(1986), pp. 28-37.

Assignment of phosphorylation sites in buffalo beta-casein by fast atom bombardment mass spectrometry.

PETRILLI, PASQUALE;PUCCI, PIETRO;ADDEO, FRANCESCO
1986

Abstract

Fast atom bombardment mass spectrometry has been applied to the localization of phosphorylation sites in buffalo beta-casein. Two complementary strategies of identification are described. Phosphorylated residues in the tryptic peptide Tp 1 have been assigned by measuring the masses of peptide fragments obtained by enzymatic degradations. The phosphoserine residue in peptide Tp 2 has been identified by determining the intact molecular weight and confirmed by partial sequence information. This rapid and sensitive procedure appears of a great interest in structural studies of a wide range of post-translational modifications in proteins.
1986
Assignment of phosphorylation sites in buffalo beta-casein by fast atom bombardment mass spectrometry / Petrilli, Pasquale; Pucci, Pietro; Morris, Hr; Addeo, Francesco. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 140:1(1986), pp. 28-37.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/356130
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