Carnitine is a molecule with well-documented pleiotropic functions whose biosynthesis involves four catalytic steps. Here, we report a detailed analysis of the expression and transcriptional control of TMLH gene, which codifies for the first enzyme of carnitine biosynthesis. TMLH maps at the extreme end of Xq28, a chromosomal region of high genomic instability. By 5′ and 3′ RACE, we identified and mapped two alternative 5′ TMLH first exons and seven alternative 3′-splice variants, which are spread over a genomic region of about 250 kb. While the two alternative 5′ exons have different expression profiles, all the 3′ alternative forms are ubiquitously expressed. Reporter assays revealed that the 3′-UTRs of each TMLH isoform might influence its own expression at post-transcriptional level. In addition, we identified a highly conserved promoter region of TMLH. Functional analysis of this region showed the presence of a CpG island, whose methylation-status could control the level of TMLH transcription. Finally, by mRNA in situ hybridization, we found that TMLH expression is present at E12.5 dpc in the mouse liver, lung and brain, and is then maintained in the postnatal brain with a specific neuronal pattern. Collectively, our data highlight a tight transcriptional and posttranscriptional control of TMLH expression.

Funcional characterization of the TMLH gene: promoter analysis, in situ hybridization, identification and mapping of alternative splicing variants / J., Monfregola; G., Napolitano; I., Conte; Cevenini, Armando; C., Migliaccio; M., D'Urso; M. V., Ursini; Napolitano, Gennaro; Conte, Ivan. - In: GENE. - ISSN 0378-1119. - STAMPA. - 395:1-2(2007), pp. 86-97. [10.1016/j.gene.2007.02.012]

Funcional characterization of the TMLH gene: promoter analysis, in situ hybridization, identification and mapping of alternative splicing variants

CEVENINI, Armando;NAPOLITANO, GENNARO;CONTE, IVAN
2007

Abstract

Carnitine is a molecule with well-documented pleiotropic functions whose biosynthesis involves four catalytic steps. Here, we report a detailed analysis of the expression and transcriptional control of TMLH gene, which codifies for the first enzyme of carnitine biosynthesis. TMLH maps at the extreme end of Xq28, a chromosomal region of high genomic instability. By 5′ and 3′ RACE, we identified and mapped two alternative 5′ TMLH first exons and seven alternative 3′-splice variants, which are spread over a genomic region of about 250 kb. While the two alternative 5′ exons have different expression profiles, all the 3′ alternative forms are ubiquitously expressed. Reporter assays revealed that the 3′-UTRs of each TMLH isoform might influence its own expression at post-transcriptional level. In addition, we identified a highly conserved promoter region of TMLH. Functional analysis of this region showed the presence of a CpG island, whose methylation-status could control the level of TMLH transcription. Finally, by mRNA in situ hybridization, we found that TMLH expression is present at E12.5 dpc in the mouse liver, lung and brain, and is then maintained in the postnatal brain with a specific neuronal pattern. Collectively, our data highlight a tight transcriptional and posttranscriptional control of TMLH expression.
2007
Funcional characterization of the TMLH gene: promoter analysis, in situ hybridization, identification and mapping of alternative splicing variants / J., Monfregola; G., Napolitano; I., Conte; Cevenini, Armando; C., Migliaccio; M., D'Urso; M. V., Ursini; Napolitano, Gennaro; Conte, Ivan. - In: GENE. - ISSN 0378-1119. - STAMPA. - 395:1-2(2007), pp. 86-97. [10.1016/j.gene.2007.02.012]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/344811
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