HMGA2 protein was detectable in four out of 21 follicular carcinomas, 30 out of 45 papillary carcinomas, and 11 out of 12 undifferentiated carcinomas. As far as follicular thyroid adenomas are concerned, only three cases of the 31 analysed showed HMGA2 protein expression, whereas it was absent in seven normal thyroid tissues and in 12 hyperplastic nodules. Quantitative RT-PCR showed that almost all the papillary thyroid carcinomas and 13 out of 16 follicular thyroid carcinomas express much higher HMGA2 specific mRNA levels in comparison to normal thyroids and adenomas. Therefore, our data support the quantitative RT-PCR analysis of HMGA2 expression, rather than immunohistochemistry, as a powerful tool for the diagnosis of thyroid neoplasias.
HMGA2 mRNA expression correlates with the malignant phenotype in human thyroid neoplasias / Chiappetta, G; Ferraro, Angelo; Vuttariello, E; Monaco, M; Galdiero, F; De Simone, V; Califano, D; P., Pallante; Botti, G; Pezzullo, L; Pierantoni, GIOVANNA MARIA; Santoro, Massimo; Fusco, Alfredo. - In: EUROPEAN JOURNAL OF CANCER. - ISSN 0959-8049. - STAMPA. - 44:7(2008), pp. 1015-1021. [10.1016/j.ejca.2008.02.039]
HMGA2 mRNA expression correlates with the malignant phenotype in human thyroid neoplasias.
FERRARO, ANGELO;PIERANTONI, GIOVANNA MARIA;SANTORO, MASSIMO;FUSCO, ALFREDO
2008
Abstract
HMGA2 protein was detectable in four out of 21 follicular carcinomas, 30 out of 45 papillary carcinomas, and 11 out of 12 undifferentiated carcinomas. As far as follicular thyroid adenomas are concerned, only three cases of the 31 analysed showed HMGA2 protein expression, whereas it was absent in seven normal thyroid tissues and in 12 hyperplastic nodules. Quantitative RT-PCR showed that almost all the papillary thyroid carcinomas and 13 out of 16 follicular thyroid carcinomas express much higher HMGA2 specific mRNA levels in comparison to normal thyroids and adenomas. Therefore, our data support the quantitative RT-PCR analysis of HMGA2 expression, rather than immunohistochemistry, as a powerful tool for the diagnosis of thyroid neoplasias.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
