Bovine seminal ribonuclease is a unique case of protein dimorphism, since it exists in two dimeric forms, with di¡erent biological and kinetic behavior, which interconvert into one another through three-dimensional swapping. Here we report the crystal structure, at 2.2 A! resolution, of the unswapped form of bovine seminal ribonuclease. Besides completing the structural de¢nition of bovine seminal ribonuclease conformational dimorphism, this study provides the structural basis to explain the dependence of the enzyme cooperative e¡ects on its swapping state.
Crystal Structure of the Dimeric unswapped Form of Bovine Seminal Ribonuclease / Berisio, R; Sica, Filomena; DE LORENZO, Claudia; DI FIORE, A; Piccoli, Renata; Zagari, Adriana; Mazzarella, Lelio. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 554:(2003), pp. 105-110.
Crystal Structure of the Dimeric unswapped Form of Bovine Seminal Ribonuclease
SICA, FILOMENA;DE LORENZO, CLAUDIA;PICCOLI, RENATA;ZAGARI, ADRIANA;MAZZARELLA, LELIO
2003
Abstract
Bovine seminal ribonuclease is a unique case of protein dimorphism, since it exists in two dimeric forms, with di¡erent biological and kinetic behavior, which interconvert into one another through three-dimensional swapping. Here we report the crystal structure, at 2.2 A! resolution, of the unswapped form of bovine seminal ribonuclease. Besides completing the structural de¢nition of bovine seminal ribonuclease conformational dimorphism, this study provides the structural basis to explain the dependence of the enzyme cooperative e¡ects on its swapping state.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
