Cold environments are predominant over the Earth and are inhabited by bacteria able to cope with a series of simultaneous environmental pressures. Gram-negative species of the Pseudomonas genus are the predominant ones isolated from cold habitats, making them an excellent model for studying the mechanisms of bacterial adaptation to the most extreme habitats on our planet. Here we focused on the lipopolysaccharide (LPS) structure and the outer membrane dynamics of Pseudomonas sp. EW#7, a strain isolated from Enigma Lake in Antarctica where, among other extreme characteristics, water temperature can reach 0.4 °C. We show that near-zero growth temperature mostly affects the LPS lipid A component. An uncommon tendency of decreasing lipid A secondary hydroxylation while increasing its phosphorylation degree was observed. This resulted in a faster lateral diffusion of lipid chains in the membrane and therefore in an enhancement of its fluctuations that guarantee membrane integrity and flexibility.

Bucking the trend: understanding lipopolysaccharide structure and outer membrane dynamics in cold-adapted Pseudomonas isolated from Enigma Lake, Antarctica / Mercogliano, Marcello; De Chiara, Stefania; De Nicola, Antonio; Cardellini, Jacopo; Montis, Costanza; Yakimov, Mikhail M.; La Cono, Violetta; Crisafi, Francesca; Silipo, Alba; Berti, Debora; Milano, Giuseppe; Molinaro, Antonio; Di Lorenzo, Flaviana. - In: CHEMICAL SCIENCE. - ISSN 2041-6520. - 15:(2024), pp. 17852-17861. [10.1039/d4sc05116e]

Bucking the trend: understanding lipopolysaccharide structure and outer membrane dynamics in cold-adapted Pseudomonas isolated from Enigma Lake, Antarctica

Mercogliano, Marcello;De Chiara, Stefania;De Nicola, Antonio;Crisafi, Francesca;Silipo, Alba;Berti, Debora;Milano, Giuseppe;Molinaro, Antonio;Di Lorenzo, Flaviana
2024

Abstract

Cold environments are predominant over the Earth and are inhabited by bacteria able to cope with a series of simultaneous environmental pressures. Gram-negative species of the Pseudomonas genus are the predominant ones isolated from cold habitats, making them an excellent model for studying the mechanisms of bacterial adaptation to the most extreme habitats on our planet. Here we focused on the lipopolysaccharide (LPS) structure and the outer membrane dynamics of Pseudomonas sp. EW#7, a strain isolated from Enigma Lake in Antarctica where, among other extreme characteristics, water temperature can reach 0.4 °C. We show that near-zero growth temperature mostly affects the LPS lipid A component. An uncommon tendency of decreasing lipid A secondary hydroxylation while increasing its phosphorylation degree was observed. This resulted in a faster lateral diffusion of lipid chains in the membrane and therefore in an enhancement of its fluctuations that guarantee membrane integrity and flexibility.
2024
Bucking the trend: understanding lipopolysaccharide structure and outer membrane dynamics in cold-adapted Pseudomonas isolated from Enigma Lake, Antarctica / Mercogliano, Marcello; De Chiara, Stefania; De Nicola, Antonio; Cardellini, Jacopo; Montis, Costanza; Yakimov, Mikhail M.; La Cono, Violetta; Crisafi, Francesca; Silipo, Alba; Berti, Debora; Milano, Giuseppe; Molinaro, Antonio; Di Lorenzo, Flaviana. - In: CHEMICAL SCIENCE. - ISSN 2041-6520. - 15:(2024), pp. 17852-17861. [10.1039/d4sc05116e]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/977196
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