: Multiple sclerosis (MS) is an autoimmune chronic disease characterized by inflammation and demyelination of the central nervous system (CNS). Despite numerous studies conducted, valid biomarkers enabling a definitive diagnosis of MS are not yet available. The aim of our study was to identify a marker from a blood sample to ease the diagnosis of MS. In this study, since there is evidence connecting the serotonin pathway to MS, we used an ELISA (Enzyme-Linked Immunosorbent Assay) to detect serum MS-specific auto-antibodies (auto-Ab) against the extracellular loop 1 (ECL-1) of the 5-hydroxytryptamine (5-HT) receptor subtype 2A (5-HT2A). We utilized an ELISA format employing poly-D-lysine as a pre-coating agent. The binding of 208 serum samples from controls, both healthy and pathological, and of 104 serum samples from relapsing-remitting MS (RRMS) patients was tested. We observed that the serum-binding activity in control cohort sera, including those with autoimmune and neurological diseases, was ten times lower compared to the RRMS patient cohort (p = 1.2 × 10-47), with a sensitivity and a specificity of 98% and 100%, respectively. These results show that in the serum of patients with MS there are auto-Ab against the serotonin receptor type 2A which can be successfully used in the diagnosis of MS due to their high sensitivity and specificity.

A Blood Test for the Diagnosis of Multiple Sclerosis / Giuliano, Paola; La Rosa, Giuliana; Capozzi, Serena; Cassano, Emanuele; Damiano, Simona; Habetswallner, Francesco; Marra, Maurizio; Pavone, Luigi Michele; Quarantelli, Mario; Vitelli, Giuseppe; Santillo, Mariarosaria; Paternò, Roberto; Iodice, Rosa. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 25:3(2024). [10.3390/ijms25031696]

A Blood Test for the Diagnosis of Multiple Sclerosis

Giuliano, Paola;La Rosa, Giuliana;Capozzi, Serena;Cassano, Emanuele;Damiano, Simona;Habetswallner, Francesco;Marra, Maurizio;Pavone, Luigi Michele;Santillo, Mariarosaria;
2024

Abstract

: Multiple sclerosis (MS) is an autoimmune chronic disease characterized by inflammation and demyelination of the central nervous system (CNS). Despite numerous studies conducted, valid biomarkers enabling a definitive diagnosis of MS are not yet available. The aim of our study was to identify a marker from a blood sample to ease the diagnosis of MS. In this study, since there is evidence connecting the serotonin pathway to MS, we used an ELISA (Enzyme-Linked Immunosorbent Assay) to detect serum MS-specific auto-antibodies (auto-Ab) against the extracellular loop 1 (ECL-1) of the 5-hydroxytryptamine (5-HT) receptor subtype 2A (5-HT2A). We utilized an ELISA format employing poly-D-lysine as a pre-coating agent. The binding of 208 serum samples from controls, both healthy and pathological, and of 104 serum samples from relapsing-remitting MS (RRMS) patients was tested. We observed that the serum-binding activity in control cohort sera, including those with autoimmune and neurological diseases, was ten times lower compared to the RRMS patient cohort (p = 1.2 × 10-47), with a sensitivity and a specificity of 98% and 100%, respectively. These results show that in the serum of patients with MS there are auto-Ab against the serotonin receptor type 2A which can be successfully used in the diagnosis of MS due to their high sensitivity and specificity.
2024
A Blood Test for the Diagnosis of Multiple Sclerosis / Giuliano, Paola; La Rosa, Giuliana; Capozzi, Serena; Cassano, Emanuele; Damiano, Simona; Habetswallner, Francesco; Marra, Maurizio; Pavone, Luigi Michele; Quarantelli, Mario; Vitelli, Giuseppe; Santillo, Mariarosaria; Paternò, Roberto; Iodice, Rosa. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 25:3(2024). [10.3390/ijms25031696]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/959022
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