: Peptide-based hydrogels have been recently investigated as materials for biomedical applications like tissue engineering and delivery of drugs and imaging agents. Among the synthetic peptide hydrogelators, the cationic hexapeptides Ac-K1 and Ac-K2 were proposed as scaffolds for bioprinting applications. Here, we report the formulation of Ac-K1 and Ac-K2 hydrogels loaded with iopamidol, an iodinated contrast agent clinically approved for X-ray computed tomography, and more recently identified as an efficient CEST-MRI probe. Iopamidol-loaded hydrogels were soft, injectable and non-toxic both in vitro (on three tumor cell lines: GL261, TS/A and 3T3-NIH) and in vivo (in Balb/c mice inoculated with TS/A breast cancer cells). The in vitro CEST-MRI study evidenced the typical features of the CEST pattern of iopamidol, with a CEST contrast higher than 50%. Due to their injectability and good ability to retain the contrast agent, the herein investigated systems can be considered as promising candidates for the development of smart MRI detectable hydrogels.

Development of cationic peptide-based hydrogels loaded with iopamidol for CEST-MRI detection / Di Gregorio, Enza; Rosa, Elisabetta; Ferrauto, Giuseppe; Diaferia, Carlo; Gallo, Enrico; Accardo, Antonella; Terreno, Enzo. - In: JOURNAL OF MATERIALS CHEMISTRY. B. - ISSN 2050-750X. - 11:31(2023), pp. 7435-7441. [10.1039/D3TB00187C]

Development of cationic peptide-based hydrogels loaded with iopamidol for CEST-MRI detection

Rosa, Elisabetta;Diaferia, Carlo;Gallo, Enrico;Accardo, Antonella
;
2023

Abstract

: Peptide-based hydrogels have been recently investigated as materials for biomedical applications like tissue engineering and delivery of drugs and imaging agents. Among the synthetic peptide hydrogelators, the cationic hexapeptides Ac-K1 and Ac-K2 were proposed as scaffolds for bioprinting applications. Here, we report the formulation of Ac-K1 and Ac-K2 hydrogels loaded with iopamidol, an iodinated contrast agent clinically approved for X-ray computed tomography, and more recently identified as an efficient CEST-MRI probe. Iopamidol-loaded hydrogels were soft, injectable and non-toxic both in vitro (on three tumor cell lines: GL261, TS/A and 3T3-NIH) and in vivo (in Balb/c mice inoculated with TS/A breast cancer cells). The in vitro CEST-MRI study evidenced the typical features of the CEST pattern of iopamidol, with a CEST contrast higher than 50%. Due to their injectability and good ability to retain the contrast agent, the herein investigated systems can be considered as promising candidates for the development of smart MRI detectable hydrogels.
2023
Development of cationic peptide-based hydrogels loaded with iopamidol for CEST-MRI detection / Di Gregorio, Enza; Rosa, Elisabetta; Ferrauto, Giuseppe; Diaferia, Carlo; Gallo, Enrico; Accardo, Antonella; Terreno, Enzo. - In: JOURNAL OF MATERIALS CHEMISTRY. B. - ISSN 2050-750X. - 11:31(2023), pp. 7435-7441. [10.1039/D3TB00187C]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/951962
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