Agri-food residues offer significant potential as a raw material for the production of L-lactic acid through microbial fermentation. Weizmannia coagulans, previously known as Bacillus coagulans, is a spore-forming, lactic acid-producing, gram-positive, with known probiotic and prebiotic properties. This study aimed to evaluate the feasibility of utilizing untreated citrus waste as a sustainable feedstock for the production of L-lactic acid in a one-step process, by using the strain W. coagulans MA-13. By employing a thermophilic enzymatic cocktail (Cellic CTec2) in conjunction with the hydrolytic capabilities of MA-13, biomass degradation was enhanced by up to 62%. Moreover, batch and fed-batch fermentation experiments demonstrated the complete fermentation of glucose into L-lactic acid, achieving a concentration of up to 44.8 g/L. These results point to MA-13 as a microbial cell factory for one-step production of L-lactic acid, by combining cost-effective saccharification with MA-13 fermentative performance, on agri-food wastes. Moreover, the potential of this approach for sustainable valorization of agricultural waste streams is successfully proven. Key points • Valorization of citrus waste, an abundant residue in Mediterranean countries. • Sustainable production of the L-( +)-lactic acid in one-step process. • Enzymatic pretreatment is a valuable alternative to the use of chemical. Graphical Abstract

Thermophilic biocatalysts for one-step conversion of citrus waste into lactic acid / Aulitto, Martina; Alfano, Alberto; Maresca, Emanuela; Avolio, Roberto; Emanuela Errico, Maria; Gentile, Gennaro; Cozzolino, Flora; Monti, Maria; Pirozzi, Annachiara; Donsì, Francesco; Cimini, Donatella; Schiraldi, Chiara; Contursi, Patrizia. - In: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. - ISSN 0175-7598. - 108:1(2024), p. 155. [10.1007/s00253-023-12904-7]

Thermophilic biocatalysts for one-step conversion of citrus waste into lactic acid

Martina Aulitto
Co-primo
;
Alberto Alfano
Co-primo
;
Emanuela Maresca;Roberto Avolio;Flora Cozzolino;Maria Monti;Patrizia Contursi
2024

Abstract

Agri-food residues offer significant potential as a raw material for the production of L-lactic acid through microbial fermentation. Weizmannia coagulans, previously known as Bacillus coagulans, is a spore-forming, lactic acid-producing, gram-positive, with known probiotic and prebiotic properties. This study aimed to evaluate the feasibility of utilizing untreated citrus waste as a sustainable feedstock for the production of L-lactic acid in a one-step process, by using the strain W. coagulans MA-13. By employing a thermophilic enzymatic cocktail (Cellic CTec2) in conjunction with the hydrolytic capabilities of MA-13, biomass degradation was enhanced by up to 62%. Moreover, batch and fed-batch fermentation experiments demonstrated the complete fermentation of glucose into L-lactic acid, achieving a concentration of up to 44.8 g/L. These results point to MA-13 as a microbial cell factory for one-step production of L-lactic acid, by combining cost-effective saccharification with MA-13 fermentative performance, on agri-food wastes. Moreover, the potential of this approach for sustainable valorization of agricultural waste streams is successfully proven. Key points • Valorization of citrus waste, an abundant residue in Mediterranean countries. • Sustainable production of the L-( +)-lactic acid in one-step process. • Enzymatic pretreatment is a valuable alternative to the use of chemical. Graphical Abstract
2024
Thermophilic biocatalysts for one-step conversion of citrus waste into lactic acid / Aulitto, Martina; Alfano, Alberto; Maresca, Emanuela; Avolio, Roberto; Emanuela Errico, Maria; Gentile, Gennaro; Cozzolino, Flora; Monti, Maria; Pirozzi, Annachiara; Donsì, Francesco; Cimini, Donatella; Schiraldi, Chiara; Contursi, Patrizia. - In: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY. - ISSN 0175-7598. - 108:1(2024), p. 155. [10.1007/s00253-023-12904-7]
File in questo prodotto:
File Dimensione Formato  
s00253-023-12904-7.pdf

accesso aperto

Licenza: Dominio pubblico
Dimensione 1.36 MB
Formato Adobe PDF
1.36 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/951490
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 4
  • ???jsp.display-item.citation.isi??? 2
social impact