Optimizing the experimental layout and the computational processing for lab-on-chip based tomographic flow cytometry system

A quasi-common-path lateral-shearing holo-tomographic optical setup coupled to microfluidic channel is designed and build-up. We demonstrate that 3D refractive index profile of flowing cells can be efficiently retrieved. This setup is robust to external vibrations and is scaled down in dimension, in the perspective of employing such systems outside of optical labs. We apply a reconstruction algorithm based on high-order total-variation to cope with the reduced quality of the measurements acquired. Validation of such reconstruction approach is performed. The assessment of the robustness of the proposed solution is allowed by evaluating some biophysical parameters of biological relevance over the reconstructed tomograms.