Pluripotent stem cells are undifferentiated cells with the extraordinary capability to differentiate into all cellular types. Based on these characteristics, human induced pluripotent cells (hiPSCs), produced in the laboratory, represent the new frontier of regenerative medicine as an innovative platform for partial or complete regeneration of injured tissues. The metabolic analysis of hiPSCs is fundamental in understanding the mechanisms underlying their functioning and ability to respond to particular environmental conditions, as well as how to develop better strategies for their production. In this context, genome-scale metabolic models (GEMs) offer a powerful tool to model and investigate the metabolism of hiPSCs. In this work, we present hiPSCGEM01, the first version of the context-specific genome-scale metabolic model (GEM) for the hiPSCs, extracted from RECON 3D, [1], as well as a first analysis of the pathways and essential genes reconstructed in the iPSCs specific model. Future developments will focus on the metabolic analysis of the phenotypes resulting from the knockout of the identified essential genes and on the possibility of exploiting the devised metabolic model to design new optimal culture media.
A preliminary version of a Genome-Scale Metabolic Model for Induced Human Pluripotent Stem Cells (hiPSCs) / Procopio, A.; Parrotta, E.; Scalise, S.; Cortese, N.; Merola, A.; Amato, F.; Cuda, G.; Cosentino, C.. - (2023). (Intervento presentato al convegno Convegno Nazionale di Bioingegneria 2023 tenutosi a Padova nel 21-23 giugno 2023).
A preliminary version of a Genome-Scale Metabolic Model for Induced Human Pluripotent Stem Cells (hiPSCs)
Amato F.;
2023
Abstract
Pluripotent stem cells are undifferentiated cells with the extraordinary capability to differentiate into all cellular types. Based on these characteristics, human induced pluripotent cells (hiPSCs), produced in the laboratory, represent the new frontier of regenerative medicine as an innovative platform for partial or complete regeneration of injured tissues. The metabolic analysis of hiPSCs is fundamental in understanding the mechanisms underlying their functioning and ability to respond to particular environmental conditions, as well as how to develop better strategies for their production. In this context, genome-scale metabolic models (GEMs) offer a powerful tool to model and investigate the metabolism of hiPSCs. In this work, we present hiPSCGEM01, the first version of the context-specific genome-scale metabolic model (GEM) for the hiPSCs, extracted from RECON 3D, [1], as well as a first analysis of the pathways and essential genes reconstructed in the iPSCs specific model. Future developments will focus on the metabolic analysis of the phenotypes resulting from the knockout of the identified essential genes and on the possibility of exploiting the devised metabolic model to design new optimal culture media.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
