: Lipidation of the LC3 protein has frequently been employed as a marker of autophagy. However, LC3-lipidation is also triggered by stimuli not related to canonical autophagy. Therefore, characterization of the driving parameters for LC3 lipidation is crucial to understanding the biological roles of LC3. We identified a pseudo-natural product, termed Inducin, that increases LC3 lipidation independently of canonical autophagy, impairs lysosomal function and rapidly recruits Galectin 3 to lysosomes. Inducin treatment promotes Endosomal Sorting Complex Required for Transport (ESCRT)-dependent membrane repair and transcription factor EB (TFEB)-dependent lysosome biogenesis ultimately leading to cell death.
Inducin Triggers LC3‐Lipidation and ESCRT‐Mediated Lysosomal Membrane Repair / Corkery, Dale; Ursu, Andrei; Lucas, Belén; Grigalunas, Michael; Kriegler, Simon; Oliva, Rosario; Dec, Robert; Koska, Sandra; Pahl, Axel; Sievers, Sonja; Ziegler, Slava; Winter, Roland; Wu, Yao‐wen; Waldmann, Herbert. - In: CHEMBIOCHEM. - ISSN 1439-4227. - (2023), p. e202300579. [10.1002/cbic.202300579]
Inducin Triggers LC3‐Lipidation and ESCRT‐Mediated Lysosomal Membrane Repair
Rosario Oliva;
2023
Abstract
: Lipidation of the LC3 protein has frequently been employed as a marker of autophagy. However, LC3-lipidation is also triggered by stimuli not related to canonical autophagy. Therefore, characterization of the driving parameters for LC3 lipidation is crucial to understanding the biological roles of LC3. We identified a pseudo-natural product, termed Inducin, that increases LC3 lipidation independently of canonical autophagy, impairs lysosomal function and rapidly recruits Galectin 3 to lysosomes. Inducin treatment promotes Endosomal Sorting Complex Required for Transport (ESCRT)-dependent membrane repair and transcription factor EB (TFEB)-dependent lysosome biogenesis ultimately leading to cell death.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
