The urokinase-type plasminogen activator (uPA) is a specific protease which, by converting plasminogen into plasmin, can promote the degradation of the extracellular matrix. uPA and its specific cellular receptor (uPA-R) are involved in cell migration and in tissue remodelling process. The urokinase-type plasminogen-activator receptor (uPA-R) cross-linked to its possible ligands, in THP-1 cells, appears to have a molecular wheight 10-15 kDa higher than the same molecular species in the epithelial thyroid cell line TAD-2. The same difference is observed comparing peripheral monocytes and primary cultures of thyroid. Molecular wheight of not cross-linked receptors is about 50 kDa and appears to be identical in these two cell types. These results suggest that, in monocytic cells, uPA-R associates with a 10-15 kDa molecule. This molecule is probably linked to the cell surface by a glyco-phospho-inositol anchor because it is eluted with the urokinase-type plasminogen activator receptor from THP-1 cells by phospholipase-C treatment.
Urokinase-type plasminogek-activator receptor associates with a cell surface molecule in monocytic cells / Montuori, N.; Rossi, G.. - In: FIBRINOLYSIS. - ISSN 0268-9499. - 10:3(1996), p. 34. [10.1016/s0268-9499(96)80193-7]
Urokinase-type plasminogek-activator receptor associates with a cell surface molecule in monocytic cells
Montuori N.;
1996
Abstract
The urokinase-type plasminogen activator (uPA) is a specific protease which, by converting plasminogen into plasmin, can promote the degradation of the extracellular matrix. uPA and its specific cellular receptor (uPA-R) are involved in cell migration and in tissue remodelling process. The urokinase-type plasminogen-activator receptor (uPA-R) cross-linked to its possible ligands, in THP-1 cells, appears to have a molecular wheight 10-15 kDa higher than the same molecular species in the epithelial thyroid cell line TAD-2. The same difference is observed comparing peripheral monocytes and primary cultures of thyroid. Molecular wheight of not cross-linked receptors is about 50 kDa and appears to be identical in these two cell types. These results suggest that, in monocytic cells, uPA-R associates with a 10-15 kDa molecule. This molecule is probably linked to the cell surface by a glyco-phospho-inositol anchor because it is eluted with the urokinase-type plasminogen activator receptor from THP-1 cells by phospholipase-C treatment.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
