: Exploring opportunities for biowaste valorization, herein, humic substances (HS) were combined with gelatin, a hydrophilic biocompatible and bioavailable polymer, to obtain 3D hydrogels. Hybrid gels (Gel HS) were prepared at different HS contents, exploiting physical or chemical cross-linking, through 1-ethyl-(3-3-dimethylaminopropyl)carbodiimide (EDC) chemistry, between HS and gelatin. Physicochemical features were assessed through rheological measurements, X-ray diffraction, attenuated total reflectance (ATR) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, and scanning electron microscopy (SEM). ATR and NMR spectroscopies suggested the formation of an amide bond between HS and Gel via EDC chemistry. In addition, antioxidant and antimicrobial features toward both Gram(-) and Gram(+) strains were evaluated. HS confers great antioxidant and widespread antibiotic performance to the whole gel. Furthermore, the chemical cross-linking affects the viscoelastic behavior, crystalline structures, water uptake, and functional performance and produces a marked improvement of biocide action.

Waste to Wealth Approach: Improved Antimicrobial Properties in Bioactive Hydrogels through Humic Substance-Gelatin Chemical Conjugation / Venezia, Virginia; Verrillo, Mariavittoria; Avallone, Pietro Renato; Silvestri, Brigida; Cangemi, Silvana; Pasquino, Rossana; Grizzuti, Nino; Spaccini, Riccardo; Luciani, Giuseppina. - In: BIOMACROMOLECULES. - ISSN 1525-7797. - (2023). [10.1021/acs.biomac.3c00143]

Waste to Wealth Approach: Improved Antimicrobial Properties in Bioactive Hydrogels through Humic Substance-Gelatin Chemical Conjugation

Venezia, Virginia;Verrillo, Mariavittoria;Avallone, Pietro Renato;Silvestri, Brigida;Cangemi, Silvana;Pasquino, Rossana;Grizzuti, Nino;Spaccini, Riccardo;Luciani, Giuseppina
2023

Abstract

: Exploring opportunities for biowaste valorization, herein, humic substances (HS) were combined with gelatin, a hydrophilic biocompatible and bioavailable polymer, to obtain 3D hydrogels. Hybrid gels (Gel HS) were prepared at different HS contents, exploiting physical or chemical cross-linking, through 1-ethyl-(3-3-dimethylaminopropyl)carbodiimide (EDC) chemistry, between HS and gelatin. Physicochemical features were assessed through rheological measurements, X-ray diffraction, attenuated total reflectance (ATR) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, and scanning electron microscopy (SEM). ATR and NMR spectroscopies suggested the formation of an amide bond between HS and Gel via EDC chemistry. In addition, antioxidant and antimicrobial features toward both Gram(-) and Gram(+) strains were evaluated. HS confers great antioxidant and widespread antibiotic performance to the whole gel. Furthermore, the chemical cross-linking affects the viscoelastic behavior, crystalline structures, water uptake, and functional performance and produces a marked improvement of biocide action.
2023
Waste to Wealth Approach: Improved Antimicrobial Properties in Bioactive Hydrogels through Humic Substance-Gelatin Chemical Conjugation / Venezia, Virginia; Verrillo, Mariavittoria; Avallone, Pietro Renato; Silvestri, Brigida; Cangemi, Silvana; Pasquino, Rossana; Grizzuti, Nino; Spaccini, Riccardo; Luciani, Giuseppina. - In: BIOMACROMOLECULES. - ISSN 1525-7797. - (2023). [10.1021/acs.biomac.3c00143]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/921879
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