A pixeled plasmonic metasurface (MS) has been developed as a surface-enhanced infrared absorption (SEIRA) spectroscopy substrate to monitor the denaturation process of a protein A (PA) monolayer. The different pixels of the MS have been properly engineered to monitor different regions of the electromagnetic spectrum. Specifically, these pixels have their plasmonic resonances placed in the range 1500–2000 cm−1, well matched with Amide I and Amide II vibrational bands. In particular, the SEIRA reflectance spectra of the native PA and the denatured PA have been compared, observing a redshift of about 10 cm−1 for both Amide I and Amide II groups. Moreover, the evaluated enhancement factor (up to 7 × 104) allows to reveal both the presence and the denaturation process occurring to a very low amount of PA molecules (about 3 fmoles).
Protein A Denaturation Sensing Through Surface Enhanced InfraRed Absorption (SEIRA) Spectroscopy / Di Meo, V.; Moccia, M.; Sanita, G.; Crescitelli, A.; Lamberti, A.; Galdi, V.; Rendina, I.; Esposito, E.. - 1005:(2023), pp. 184-189. (Intervento presentato al convegno 53rd Annual Meeting of the Italian Electronics Society, SIE 2022 tenutosi a ita nel 2022) [10.1007/978-3-031-26066-7_29].
Protein A Denaturation Sensing Through Surface Enhanced InfraRed Absorption (SEIRA) Spectroscopy
Lamberti A.;
2023
Abstract
A pixeled plasmonic metasurface (MS) has been developed as a surface-enhanced infrared absorption (SEIRA) spectroscopy substrate to monitor the denaturation process of a protein A (PA) monolayer. The different pixels of the MS have been properly engineered to monitor different regions of the electromagnetic spectrum. Specifically, these pixels have their plasmonic resonances placed in the range 1500–2000 cm−1, well matched with Amide I and Amide II vibrational bands. In particular, the SEIRA reflectance spectra of the native PA and the denatured PA have been compared, observing a redshift of about 10 cm−1 for both Amide I and Amide II groups. Moreover, the evaluated enhancement factor (up to 7 × 104) allows to reveal both the presence and the denaturation process occurring to a very low amount of PA molecules (about 3 fmoles).| File | Dimensione | Formato | |
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