Poly(ADPribosyl)ation is a post-translational protein modification, catalyzed by poly(ADP ribose) polymerase (PARPs) enzymes, responsible for ADP-ribose polymer synthesis (PAR) fromNAD+. PAR turnover is assured by poly(ADPR) glycohydrolase (PARGs) enzymes. In our previous study, the altered histology of zebrafish brain tissue, resulting in demyelination and neurodegeneration also with poly(ADPribosyl)ation hyperactivation, was demonstrated after aluminum (Al) exposure for 10 and 15 days. On the basis of this evidence, the aim of the present research was to study the synthesis and degradation of poly(ADP-ribose) in the brain of adult zebrafish exposed to 11 mg/L of Al for 10, 15, and 20 days. For this reason, PARP and PARG expression analyses were carried out, and ADPR polymers were synthesized and digested. The data showed the presence of different PARP isoforms, among which a human PARP1 counterpart was also expressed. Moreover, the highest PARP and PARG activity levels, responsible for the PAR production and its degradation, respectively, were measured after 10 and 15 days of exposure. We suppose that PARP activation is related to DNA damage induced by Al, while PARG activation is needed to avoid PAR accumulation, which is known to inhibit PARP and promote parthanatos. On the contrary, PARP activity decrease at longer exposure times suggests that neuronal cells could adopt the stratagem of reducing polymer synthesis to avoid energy expenditure and allow cell survival.

Synthesis and Degradation of Poly(ADP-ribose) in Zebrafish Brain Exposed to Aluminum / Bianchi, ANNA RITA; LA PIETRA, Alessandra; DE MAIO, Anna; Capriello, Teresa; Ferrandino, Ida. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 24:10(2023), p. 8766. [10.3390/ijms24108766]

Synthesis and Degradation of Poly(ADP-ribose) in Zebrafish Brain Exposed to Aluminum

Bianchi Anna Rita;La Pietra Alessandra;De Maio Anna
;
Capriello Teresa;Ferrandino Ida
2023

Abstract

Poly(ADPribosyl)ation is a post-translational protein modification, catalyzed by poly(ADP ribose) polymerase (PARPs) enzymes, responsible for ADP-ribose polymer synthesis (PAR) fromNAD+. PAR turnover is assured by poly(ADPR) glycohydrolase (PARGs) enzymes. In our previous study, the altered histology of zebrafish brain tissue, resulting in demyelination and neurodegeneration also with poly(ADPribosyl)ation hyperactivation, was demonstrated after aluminum (Al) exposure for 10 and 15 days. On the basis of this evidence, the aim of the present research was to study the synthesis and degradation of poly(ADP-ribose) in the brain of adult zebrafish exposed to 11 mg/L of Al for 10, 15, and 20 days. For this reason, PARP and PARG expression analyses were carried out, and ADPR polymers were synthesized and digested. The data showed the presence of different PARP isoforms, among which a human PARP1 counterpart was also expressed. Moreover, the highest PARP and PARG activity levels, responsible for the PAR production and its degradation, respectively, were measured after 10 and 15 days of exposure. We suppose that PARP activation is related to DNA damage induced by Al, while PARG activation is needed to avoid PAR accumulation, which is known to inhibit PARP and promote parthanatos. On the contrary, PARP activity decrease at longer exposure times suggests that neuronal cells could adopt the stratagem of reducing polymer synthesis to avoid energy expenditure and allow cell survival.
2023
Synthesis and Degradation of Poly(ADP-ribose) in Zebrafish Brain Exposed to Aluminum / Bianchi, ANNA RITA; LA PIETRA, Alessandra; DE MAIO, Anna; Capriello, Teresa; Ferrandino, Ida. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 24:10(2023), p. 8766. [10.3390/ijms24108766]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/921234
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