Objective: to assess whether immunohistochemical (IHC) algorithms used to classify the cell of origin (COO) of nodal Diffuse Large B-cell lymphoma (nDLBCL) in Germinal Center type (GCB) and non-GCB subtypes may be applied to Primary Cutaneous B-cell lymphoma (PCBCL) too, and which of these algorithms performs better on PCBCL. Design: Retrospective case control study. Setting: Pathology Department of the University Hospital “San Giovanni di Dio e Ruggi d′Aragona” Salerno, Italy. Participants: Fourteen PCBCL, including Primary Cutaneous follicle centre lymphoma (PCFCL) and primary cutaneous diffuse large B-cell lymphoma, Leg type (PCDLBCL-LT) and 14 nDLBCL were evaluated for 7-year period (January 2011 to December 2017). Primary cutaneous marginal zone cell lymphoma (PCMZL) cases were not included in the present study. Intervention: Evaluation of immunohistochemical CD10, BCL6, MUM1/IRF4, BCL2, MYC and Ki-67 expression and classification according to three different algorithms. Gene expression profiling (GEP) was performed on the same series using Lymph2Cx assay (Nanostring). The data obtained were compared and analysed. Results: All the IHC algorithms showed 13 GCB and 15 non-GCB. GEP showed 12 GCB, 12 activated B cell–type and 4 unclassified. Conclusions: The PCBCL were classifiable as GCB and non-GCB like the nDLBCL as IHC algorithms were concordant to GEP and produced the same results.
Immunohistochemical algorithms and gene expression profiling in primary cutaneous B-cell lymphoma / Cretella, P.; Peluso, A. L.; Picariello, C.; Cozzolino, I.; Triggiani, M.; Puzziello, A.; Giudice, V.; Sabbatino, F.; Ieni, A.; Zeppa, P.; Caputo, A.. - In: PATHOLOGY RESEARCH AND PRACTICE. - ISSN 0344-0338. - 231:(2022), p. 153804. [10.1016/j.prp.2022.153804]
Immunohistochemical algorithms and gene expression profiling in primary cutaneous B-cell lymphoma
Cretella P.;Peluso A. L.;Picariello C.;Cozzolino I.;Triggiani M.;Puzziello A.;Sabbatino F.;Zeppa P.;Caputo A.
2022
Abstract
Objective: to assess whether immunohistochemical (IHC) algorithms used to classify the cell of origin (COO) of nodal Diffuse Large B-cell lymphoma (nDLBCL) in Germinal Center type (GCB) and non-GCB subtypes may be applied to Primary Cutaneous B-cell lymphoma (PCBCL) too, and which of these algorithms performs better on PCBCL. Design: Retrospective case control study. Setting: Pathology Department of the University Hospital “San Giovanni di Dio e Ruggi d′Aragona” Salerno, Italy. Participants: Fourteen PCBCL, including Primary Cutaneous follicle centre lymphoma (PCFCL) and primary cutaneous diffuse large B-cell lymphoma, Leg type (PCDLBCL-LT) and 14 nDLBCL were evaluated for 7-year period (January 2011 to December 2017). Primary cutaneous marginal zone cell lymphoma (PCMZL) cases were not included in the present study. Intervention: Evaluation of immunohistochemical CD10, BCL6, MUM1/IRF4, BCL2, MYC and Ki-67 expression and classification according to three different algorithms. Gene expression profiling (GEP) was performed on the same series using Lymph2Cx assay (Nanostring). The data obtained were compared and analysed. Results: All the IHC algorithms showed 13 GCB and 15 non-GCB. GEP showed 12 GCB, 12 activated B cell–type and 4 unclassified. Conclusions: The PCBCL were classifiable as GCB and non-GCB like the nDLBCL as IHC algorithms were concordant to GEP and produced the same results.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
