: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) emerged in late December 2019 and spread worldwide, quickly becoming a pandemic. This zoonotic coronavirus shows a broad host range, including wildlife and domestic animals. Small ruminants are shown to be susceptible to SARS-CoV-2 but, to date, no natural infection has been reported. Herein, we performed a survey for SARS-CoV-2 among sheep and goats in the Campania region of Italy using an indirect multispecies ELISA. Next, positive sera were submitted to virus serum neutralization for the quantification of specific neutralizing antibodies. Out of 612 sheep and goats, 23 were found ELISA positive (3.75%) and 1 of them showed 1:20 neutralizing antibodies titer. No significant difference was found between the two species, as well as between male and female, geographical location and age. Our findings demonstrate that natural infection can occur in flocks in a field situation. Moreover, low susceptibility to SARS-CoV-2 is reported for sheep and goats, nevertheless, the continuous mutations of this virus open new scenarios on viral host range and tropism, highlighting the importance of investigating animal species that could represent ongoing or future possible hosts.

First serological evidence of SARS-CoV-2 natural infection in small ruminants : Brief report / Fusco, Giovanna; Cardillo, Lorena; Levante, Martina; Brandi, Sergio; Picazio, Gerardo; Napoletano, Michele; Martucciello, Alessandra; Fiorito, Filomena; De Carlo, Esterina; de Martinis, Claudio. - In: VETERINARY RESEARCH COMMUNICATIONS. - ISSN 0165-7380. - (2023), pp. 1-8. [10.1007/s11259-022-10044-3]

First serological evidence of SARS-CoV-2 natural infection in small ruminants : Brief report

Fusco, Giovanna;Cardillo, Lorena;Fiorito, Filomena;De Carlo, Esterina;de Martinis, Claudio
2023

Abstract

: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) emerged in late December 2019 and spread worldwide, quickly becoming a pandemic. This zoonotic coronavirus shows a broad host range, including wildlife and domestic animals. Small ruminants are shown to be susceptible to SARS-CoV-2 but, to date, no natural infection has been reported. Herein, we performed a survey for SARS-CoV-2 among sheep and goats in the Campania region of Italy using an indirect multispecies ELISA. Next, positive sera were submitted to virus serum neutralization for the quantification of specific neutralizing antibodies. Out of 612 sheep and goats, 23 were found ELISA positive (3.75%) and 1 of them showed 1:20 neutralizing antibodies titer. No significant difference was found between the two species, as well as between male and female, geographical location and age. Our findings demonstrate that natural infection can occur in flocks in a field situation. Moreover, low susceptibility to SARS-CoV-2 is reported for sheep and goats, nevertheless, the continuous mutations of this virus open new scenarios on viral host range and tropism, highlighting the importance of investigating animal species that could represent ongoing or future possible hosts.
2023
First serological evidence of SARS-CoV-2 natural infection in small ruminants : Brief report / Fusco, Giovanna; Cardillo, Lorena; Levante, Martina; Brandi, Sergio; Picazio, Gerardo; Napoletano, Michele; Martucciello, Alessandra; Fiorito, Filomena; De Carlo, Esterina; de Martinis, Claudio. - In: VETERINARY RESEARCH COMMUNICATIONS. - ISSN 0165-7380. - (2023), pp. 1-8. [10.1007/s11259-022-10044-3]
File in questo prodotto:
File Dimensione Formato  
Fusco_et_al-2023-Veterinary_Research_Communications.pdf

accesso aperto

Tipologia: Versione Editoriale (PDF)
Licenza: Dominio pubblico
Dimensione 912.97 kB
Formato Adobe PDF
912.97 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/906389
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 9
  • ???jsp.display-item.citation.isi??? 8
social impact