Phagocytosis of invading pathogens or cellular debris requires a dramatic change in cell shape driven by actin polymerization. For antibody-covered targets, phagocytosis is thought to proceed through the sequential engagement of Fc-receptors on the phagocyte with antibodies on the target surface, leading to the extension and closure of the phagocytic cup around the target. We find that two actin-dependent molecular motors, class 1 myosins myosin le and myosin if, are specifically localized to Fc-receptor adhesions and required for efficient phagocytosis of antibody-opsonized targets. Using primary macrophages lacking both myosin le and myosin if, we find that without the actin-membrane linkage mediated by these myosins, the organization of individual adhesions is compromised, leading to excessive actin polymerization, slower adhesion turnover, and deficient phagocytic internalization. This work identifies a role for class 1 myosins in coordinated adhesion turnover during phagocytosis and supports a mechanism involving membrane-cytoskeletal crosstalk for phagocytic cup closure.

Membrane-cytoskeletal crosstalk mediated by myosin-I regulates adhesion turnover during phagocytosis / Barger, Sr; Reilly, Ns; Shutova, Ms; Li, Q; Maiuri, P; Heddleston, Jm; Mooseker, Ms; Flavell, Ra; Svitkina, T; Oakes, Pw; Krendel, M; Gauthier, Nc. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - 10:(2019). [10.1038/s41467-019-09104-1]

Membrane-cytoskeletal crosstalk mediated by myosin-I regulates adhesion turnover during phagocytosis

Maiuri P;
2019

Abstract

Phagocytosis of invading pathogens or cellular debris requires a dramatic change in cell shape driven by actin polymerization. For antibody-covered targets, phagocytosis is thought to proceed through the sequential engagement of Fc-receptors on the phagocyte with antibodies on the target surface, leading to the extension and closure of the phagocytic cup around the target. We find that two actin-dependent molecular motors, class 1 myosins myosin le and myosin if, are specifically localized to Fc-receptor adhesions and required for efficient phagocytosis of antibody-opsonized targets. Using primary macrophages lacking both myosin le and myosin if, we find that without the actin-membrane linkage mediated by these myosins, the organization of individual adhesions is compromised, leading to excessive actin polymerization, slower adhesion turnover, and deficient phagocytic internalization. This work identifies a role for class 1 myosins in coordinated adhesion turnover during phagocytosis and supports a mechanism involving membrane-cytoskeletal crosstalk for phagocytic cup closure.
2019
Membrane-cytoskeletal crosstalk mediated by myosin-I regulates adhesion turnover during phagocytosis / Barger, Sr; Reilly, Ns; Shutova, Ms; Li, Q; Maiuri, P; Heddleston, Jm; Mooseker, Ms; Flavell, Ra; Svitkina, T; Oakes, Pw; Krendel, M; Gauthier, Nc. - In: NATURE COMMUNICATIONS. - ISSN 2041-1723. - 10:(2019). [10.1038/s41467-019-09104-1]
File in questo prodotto:
File Dimensione Formato  
Barger et al. - 2019 - Membrane-cytoskeletal crosstalk mediated by myosin-I regulates adhesion turnover during phagocytosis.pdf

accesso aperto

Tipologia: Versione Editoriale (PDF)
Licenza: Accesso privato/ristretto
Dimensione 11.89 MB
Formato Adobe PDF
11.89 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11588/888265
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 47
  • ???jsp.display-item.citation.isi??? 47
social impact