Management and control of the COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus SARS-CoV-2 is critically dependent on quick and reliable identification of the virus in clinical specimens. Detection of viral RNA by a colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a simple, reliable and cost-effective assay, deployable in resource-limited settings (RLS). Our objective was to evaluate the intrinsic and extrinsic performances of RT-LAMP in RLS.
Diagnostic performance of a colorimetric RT -LAMP for the identification of SARS-CoV-2: A multicenter prospective clinical evaluation in sub-Saharan Africa / Baba, Marycelin Mandu; Bitew, Molalegne; Fokam, Joseph; Lelo, Eric Agola; Ahidjo, Ahmed; Asmamaw, Kominist; Beloumou, Grace Angong; Bulimo, Wallace Dimbuson; Buratti, Emanuele; Chenwi, Collins; Dadi, Hailu; D'Agaro, Pierlanfranco; De Conti, Laura; Fainguem, Nadine; Gadzama, Galadima; Maiuri, Paolo; Majanja, Janet; Meshack, Wadegu; Ndjolo, Alexis; Nkenfou, Celine; Oderinde, Bamidele Soji; Opanda, Silvanos Mukunzi; Segat, Ludovica; Stuani, Cristiana; Symekher, Samwel L; Takou, Desire; Tesfaye, Kassahun; Triolo, Gianluca; Tuki, Keyru; Zacchigna, Serena; Marcello, Alessandro. - In: ECLINICALMEDICINE. - ISSN 2589-5370. - 40:(2021). [10.1016/j.eclinm.2021.101101]
Diagnostic performance of a colorimetric RT -LAMP for the identification of SARS-CoV-2: A multicenter prospective clinical evaluation in sub-Saharan Africa
Maiuri, Paolo;
2021
Abstract
Management and control of the COVID-19 pandemic caused by the severe acute respiratory syndrome coronavirus SARS-CoV-2 is critically dependent on quick and reliable identification of the virus in clinical specimens. Detection of viral RNA by a colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a simple, reliable and cost-effective assay, deployable in resource-limited settings (RLS). Our objective was to evaluate the intrinsic and extrinsic performances of RT-LAMP in RLS.| File | Dimensione | Formato | |
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