For the diagnosis of anti-MAG polyneuropathy the commercial ELISA manufacturer currently recommends a cut-off of 1000 Bühlmann Titer Units (BTU). We analyzed sera from 80 anti-MAG neuropathy patients and 383 controls (with other neuropathies or healthy controls) to assess the ELISA sensitivity and specificity at different thresholds. A better combination of sensitivity/specificity was found at a threshold >1500 BTU than at >1000 BTU. The best value of specificity was obtained at threshold >7000 BTU. There was a diagnostic grey area between 1500 and 7000 BTU in which the clinical phenotypes as well as electrophysiological studies need to be carefully assessed particularly to differentiate CIDP and anti-MAG neuropathy.
Sensitivity and specificity of a commercial ELISA test for anti-MAG antibodies in patients with neuropathy / Liberatore, G.; Giannotta, C.; Sajeev, B. P.; Morenghi, E.; Terenghi, F.; Gallia, F.; Doneddu, P. E.; Manganelli, F.; Cocito, D.; Filosto, M.; Antonini, G.; Cosentino, G.; Marfia, G. A.; Clerici, A. M.; Lauria, G.; Rosso, T.; Cavaletti, G.; Nobile-Orazio, E.. - In: JOURNAL OF NEUROIMMUNOLOGY. - ISSN 0165-5728. - 345:(2020), p. 577288. [10.1016/j.jneuroim.2020.577288]
Sensitivity and specificity of a commercial ELISA test for anti-MAG antibodies in patients with neuropathy
Manganelli F.;
2020
Abstract
For the diagnosis of anti-MAG polyneuropathy the commercial ELISA manufacturer currently recommends a cut-off of 1000 Bühlmann Titer Units (BTU). We analyzed sera from 80 anti-MAG neuropathy patients and 383 controls (with other neuropathies or healthy controls) to assess the ELISA sensitivity and specificity at different thresholds. A better combination of sensitivity/specificity was found at a threshold >1500 BTU than at >1000 BTU. The best value of specificity was obtained at threshold >7000 BTU. There was a diagnostic grey area between 1500 and 7000 BTU in which the clinical phenotypes as well as electrophysiological studies need to be carefully assessed particularly to differentiate CIDP and anti-MAG neuropathy.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
