Recent reports have suggested that N-arachidonoylethanolamine (anandamide) acts as an endogenous ligand for cannabinoid receptors in mammalian brain. Here we describe methods for the extraction, purification and analysis of anandamide and related N-fatty acyl-ethanolamines (NAEs). Liquidphase extraction, silica gel G column chromatography and thin-layer chromatography (TLC) were employed for sample fractionation. Three analytical high-performance liquid chromatography (HPLC) methods for purification of NAEs were developed. Finally, analyses of NAEs by gas chromatography/mass spectrometry (GC/MS) are described. The applications of these analytical methods to the identification of anandamide and related NAEs in cell cultures as well as of artifacts in biosynthetic studies are described.
Analysis of Anadamide, an Endogenus Cannabinoid Substance, and of Other Natural N-acyl-ethanolamines / Fontana, A; DI MARZO, E.; Cadas, H.; Piomelli, D.. - In: PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS. - ISSN 0952-3278. - 53:(1995), pp. 301-308. [10.1016/0952-3278(95)90130-2 0952-3278(95)90130-2]
Analysis of Anadamide, an Endogenus Cannabinoid Substance, and of Other Natural N-acyl-ethanolamines
FONTANA A;
1995
Abstract
Recent reports have suggested that N-arachidonoylethanolamine (anandamide) acts as an endogenous ligand for cannabinoid receptors in mammalian brain. Here we describe methods for the extraction, purification and analysis of anandamide and related N-fatty acyl-ethanolamines (NAEs). Liquidphase extraction, silica gel G column chromatography and thin-layer chromatography (TLC) were employed for sample fractionation. Three analytical high-performance liquid chromatography (HPLC) methods for purification of NAEs were developed. Finally, analyses of NAEs by gas chromatography/mass spectrometry (GC/MS) are described. The applications of these analytical methods to the identification of anandamide and related NAEs in cell cultures as well as of artifacts in biosynthetic studies are described.File | Dimensione | Formato | |
---|---|---|---|
1995 PLEFA_Analytical method for Anadnamide.pdf
non disponibili
Dimensione
752.53 kB
Formato
Adobe PDF
|
752.53 kB | Adobe PDF | Visualizza/Apri Richiedi una copia |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.